Urine testing

Question 1

Anabolic steroids test in urine:

Answer 1

• Mimic action of the male sex hormone testosterone and they stimulate the growth of muscles
• Excreted in urine
• Test via gas chromatography and mass spectrometry –> urine sample vapourised with a known solvent and passed along a tube, lining of tube absorbs the gases and is analysed to give a chromatogram that can be read to show the presence of drugs

Question 2

drug testing in urine:

Answer 2

• Breakdown products of drugs filtered through kidneys and traces are in urine for some time after drug used
• Sample 1 -> tested by immunoassay using monoclonal antibodies to bind to the drug / breakdown product. If positive sample 2 used
• Sample 2 -> run through gas chromatograph / mass spectrometer to confirm

Question 3

Pregnancy hormone in urine

Answer 3

hCG