What is the genome?
What is sequencing?
The entire genetic material of an organism in the nucleus of a cell (in eukaryotes)
Working out the DNA base sequence for all DNA in a cell
-many organisms have been sequenced (human genome project which took 13 years from 2003)
What is happening to sequencing methods as of late?
Improving, becoming automated
Why is it easy to sequence prokaryotic cells?
Why is this useful?
Dont contain introns
-so genome can be used to directly sequence proteins that derive from genetic code (proteome) of organism
Can identify potential antigens to use a vaccine.
Why is is difficult to sequence complex organisms?
Have introns (eukaryotes) and regulatory genes in DNA.
-not easily used to translate proteome