Is necessary before a medical technologist reports the result as RH negative
Weak D antigen
What is the use of weak D determination
To determine the presence or absence of the Du factor or weak D antigen on red cells
Weak D can be recognized most reliably by an
Indirect antihuman globulin test
Reagent used in Weak D (4)
Anti-D serum
Antihuman globulin sera
Normal saline solution (0.85% to 0.9% sodium chloride)
22% bovine serum albumin
Specimen used in weak D
Anticoagulated blood
Manner of reporting of anti-D
+
0
RH positive
RH negative / initial
Negative reaction should be further tested for the presence of weak D with the use of
Antihuman globulin test
Manner of reporting of indirect antihuman globulin test
0
+
Du negative
Du positive