Week 4

Question 1

What are the acid-fast stain reagents?
(HINT: BAM)

Answer 1

1. Basic Fuschin
2. Acidified Alcohol
3. Methelyne Blue

Question 2

What is Mycolic Acid?

Answer 2

A barrier, protecting the cells from dehydrating, as well as from phagocytosis by immune system cells in a host

Question 3

When would you use the acid-fast stain? What Example could we use for Acid fast stain?

Answer 3

to test for TB (Tuberculosis), M. Gordonae

Question 4

Steps for Acid-Fast Stain

Answer 4

1. Mycobacteria is heat fixed as a smear
2. Add primary stain Basic Fushin while the slide is heated over the heat bath
3. Decolorize with Acid Alcohol

Question 5

What color would acid-fast stains be?

Answer 5

red