This class was created by Brainscape user ASHLEY JARED DEFENSOR.
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ASHLEY JARED DEFENSOR
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Decks in this class (23)
Factors Involved in Fixation
Temperature traditional fixation 1,
Temperature for em and histochemi...,
Autotechnicon temperature 3
52
cards
Aldehyde Fixatives
Formaldehyde origin 1,
Commercial formaldehyde concentra...,
Diluted formaldehyde concentratio...
30
cards
Metallic fixatives
Used as a 4 aqueous solution not ...,
Most common metallic fixative 2,
Excellent for preservation of cel...
16
cards
Alcohol Fixatives
Rapidly denatures and precipitate...,
Can act both as fixative and dehy...,
Ideal for small tissue fragments 3
21
cards
Other Fixatives
A fixative decalcifying agent and...,
Major drawback since it is also a...,
Remedy tissue is placed in a satu...
25
cards
Decalcifying Methods
Most commonly used acids 1,
Fast acting imparts a yellow colo...,
Nitric acid remedy wash 3
39
cards
Dehydrating Agent
Recommended for routine dehydrati...,
Non toxic and fast acting 2,
For blood and tissue films and sm...
27
cards
Clearing Agents
Process of removing the dehydrati...,
Clearing agent can make tissues t...,
Clearing agent that cannot make t...
30
cards
Infiltration / Impregnantion
2 purposes of infiltration impreg...,
Easily removed from tissues hence...,
Difficult to remove from tissues ...
39
cards
Types of Microtomes
Process of cutting tissues into v...,
Cutting engine 2,
Usually prepared 4 6 um 3
28
cards
Knives, Angles, Honing, Stropping, Types of Sections, Floatation Water Bath (fix tis)
Now disposable blades are used 1,
One side of the knife is flat whi...,
Both sides of the knife are conca...
35
cards
Adhesives
To promote attachment of tissue s...,
Most commonly used adhesive uses ...,
Provides a firmer attachment than...
7
cards
Dyes According to Composition (fix)
Derived from plants and animals e...,
Extracted from bugs picric acid f...,
Vegetable dye extracted from lich...
44
cards
Special Stains in Carbohydrates
Carbohydrates are the main source...,
Acid mucopolysaccharides are the ...,
Histochemical stain that demonstr...
17
cards
Staining of Lipids
Lipid refers to all fat and fat l...,
Lipids classified into simple com...,
Soluble in organic solvents but i...
15
cards
Staining of Proteins and Nucleic Acids
Lipid refers to all fat and fat l...,
Lipids classified into simple com...,
Soluble in organic solvents but i...
15
cards
Staining of Enzymes, Pigments and Minerals, Bone Marrow and Blood Elements
Nzyme histochemistry detects earl...,
Enzymes are tissue components tha...,
Best fixative for enzymes is chil...
41
cards
Staining of Connective Tissues
Will stain reticulin fibers black 1,
Reticulin fibers 2,
For collagen 3
15
cards
Staining of Muscles and Bones
Muscles attached to the skeleton 1,
Derived from mesenchymal tissues ...,
Found only in the heart 3
15
cards
Staining of Nervous System
Nervous system divided into cns b...,
Nervous tissue usually fixed in f...,
Routine h and e stain adequate fo...
18
cards
Staining of Microorganisms
For gram positive and negative ba...,
For gram positive and negative ba...,
For afb mycobacterium 3
13
cards
Other Stains / Staining Methods (Bancroft's)
For copper 1,
For lead salts 2,
For amyloid 3
12
cards
Other Stains (Gregorios)
For connective tissues 1,
For collagen smooth muscles mitoc...,
For dna rna and differentiation o...
45
cards
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2MTAP: HP
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