Used as a 4% aqueous solution not a routine fixative for acid mucopolysaccharide and connective tissue mucin preservation
Lead fixatives
Most common metallic fixative
Mercuric chloride fixatives
Excellent for preservation of cell details in tissue photography fixative of choice trichrome staining BEST most compatible
Mercuric chloride fixatives
Used for hematopoietic tissues reticuloendothelial tissues renal tissues connective tissue fibrins muscle tissues
Mercuric chloride fixatives
May form black mercury deposits remedy wash tissues with alcoholic iodine
Mercuric chloride fixatives
Contains Mercuric Chloride Glacial Acetic Acid Potassium Dichromate for preserving liver spleen CT fibers nuclei
Zenker’s
Contains Mercuric chloride Potassium dichromate Formalin for BM pituitary gland blood forming organs intercalated discs of cardiac muscles
Zenker Formol Helly’s Fluid
May produce brown pigment remove with saturated alcoholic picric acid or sodium hydroxide
Zenker Formol Helly’s Fluid
Contains Tricarboxylic Acid Glacial Acetic Acid Formalin Mercuric Chloride recommended for tumor skin biopsies excellent cytologic fixative
Heidenhain Susa
Contains Mercuric Chloride Anhydrous Sodium Acetate for preserving bone marrow
Lillie’s B5
Other mercuric chloride fixatives
Carnoy-Lebrun Schaudinn’s Ohlmacher’s
All contain potassium dichromate oxidizing agents for secondary fixation post-chromatization
Chromate fixatives
For carbohydrates 1-2%
Chromic Acid
For lipids and mitochondria 3%
Potassium Dichromate
For chromatin mitochondria mitotic figures golgi bodies RBC-containing colloid tissues
Regaud’s Muller’s Moller’s
For early degenerative processes tissue necrosis preserving Rickettsia other bacteria
Orth’s Fluid