Clearing Agents

Question 1

Process of removing the dehydrating agent from tissues to replace it with a medium that is miscible with both paraffin wax and the mounting medium

Answer 1

Clearing / Dealcoholization

Question 2

Clearing agent can make tissues translucent but not all clearing agents are clearers

Answer 2

Clearing agents

Question 3

Clearing agent that cannot make tissues translucent

Answer 3

Chloroform

Question 4

Commonly used as it is fast-acting with rapid clearing time of 30 minutes to hours

Answer 4

Xylene

Question 5

Not suitable for nervous tissues and lymph nodes

Answer 5

Xylene

Question 6

Becomes milky when an incompletely dehydrated tissue is immersed

Answer 6

Xylene

Question 7

Substitute for xylene and benzene clears within 1-2 hours

Answer 7

Toluene

Question 8

Acidifies in a partially-filled vessel container must be full to prevent pH changes

Toxic upon prolonged exposure

Answer 8

Toluene

Question 9

Suited for urgent biopsies highly flammable and carcinogenic

Answer 9

Benzene

Question 10

Prolonged exposure can damage the BM leading to aplastic anemia

Answer 10

Benzene

Question 11

Does not make tissues hard and brittle there is minimum shrinkage

Answer 11

Benzene

Question 12

For tough tissues skin fibroid and decalcified tissues nervous tissues lymph nodes and embryos

Answer 12

Chloroform

Question 13

Tissues tend to float remedy cover the tissue with gauze acts as a weight

Answer 13

Chloroform

Question 14

Toxic to the liver tissues do not become translucent

Answer 14

Chloroform

Question 15

Clearing time takes 2-3 days for cytological studies like smooth muscles of the skin and CNS tissue

Answer 15

Cedarwood oil

Question 16

Can become milky on prolonged storage

Answer 16

Cedarwood oil

Question 17

Not for routine purposes slow-acting recommended for clearing insects embryos and other delicate specimens

Answer 17

Aniline oil

Question 18

Recommended for smooth muscles and skin causes minimum shrinkage not recommended for routine purposes

Answer 18

Clove oil

Question 19

Clearing agent from citrus fruits and a xylene substitute

Answer 19

Limonine

Question 20

Substitute for xylene but solidifies at a low temperature

Answer 20

Coconut oil

Question 21

Similar to chloroform but cheaper toxic on prolonged exposure and can cause tissue hardening

Answer 21

Carbon tetrachloride

Question 22

Becomes milky upon contact with incompletely dehydrated tissue

Answer 22

Xylene

Question 23

Becomes milky upon prolonged storage

Answer 23

Cedarwood oil

Question 24

Slow acting clearing agents used for double embedding

Answer 24

Methyl salicylate and Methyl benzoate

Question 25

For skin and smooth muscles

Answer 25

Oil of Bergamot

Question 26

For delicate tissues

Answer 26

Oil of Wintergreen

Question 27

For smooth muscles

Answer 27

Carbon Disulfide and Phenol

Question 28

For friable tissues

Answer 28

Carbon Xylene

Question 29

For eye specimens

Answer 29

Terpineol

Question 30

Excellent clearing agent

Answer 30

High Test Aviation Lead Free Gasoline