216 prep

Question 1

Histidine structure

Answer 1

CH2, pentagon with DB at 1 and 3. NH+ at C2, NH at C4

Question 2

If pH > pI, protein is X charged.

Answer 2

negatively

Question 3

Hydrogen bonding bonds x and X

Answer 3

C=O and N-H0 directly under

Question 4

AAs that’ll have a pKa contributing to net charge

Answer 4

DRECKY + H

Question 5

How to find net charge of ptotein afer boiling in SDS

Answer 5

Divide number of AAs by 2, multiply by negative one. Add result to net charge

Question 6

What moves fastest in SDS PAGE?

Answer 6

Smallest molecules

Question 7

pKa for Tyrosine

Answer 7

10.5 (Phenol)

Question 8

pKa for Cysteine

Answer 8

8.4 (SH)

Question 9

pKa for Lysine

Answer 9

10.5 (NH3+)

Question 10

pKa for Arginine

Answer 10

12.5- Guanidino)

Question 11

pKa for Histidine

Answer 11

6 (Imidaole

Question 12

pKa for glutamic acid

Answer 12

4.1 (Gamma COOH)

Question 13

pKa for Aspartic acid

Answer 13

3.9 (COOH)

Question 14

If pH < pI, protein is X charged.

Answer 14

positively

Question 15

Arginine structure

Answer 15

3C, NH, C with 2 NH2 on it. (One with a db an + charge)

Question 16

Glutamic acid structure

Answer 16

CH2CH2COOH

Question 17

isoelectric point

Answer 17

pH where molecules have no electrical charge

Question 18

What protein should have the highest mass to charge ratio?

Answer 18

All should be about the same

Question 19

After hitting pKa, D is…

Answer 19

Negative

Question 20

After hitting pKa, R is…

Answer 20

neutral

Question 21

After hitting pKa, E is…

Answer 21

Negative

Question 22

After hitting pKa, C is…

Answer 22

positive

Question 23

After hitting pKa, K is…

Answer 23

neutral

Question 24

After hitting pKa, Y is…

Answer 24

negative

Question 25

After hitting pKa, H is...

Answer 25

positive