Lysine and arginine are X after deprotonation
Neutral
Cysteine is X after deprotonation
Negative
Where alpha helices bind
The backbone N-H of the nth residue
H-bonds with the backbone C=O of the
X residue.
Chemical and material proteins we can use to purify proteins- why do these work?
Hydrophobicity, molecular weight, net charge. Usually pretty unique to proteins
Which AAs are hydrophobic
“Grandma Always Visits London In May For Winston’s Party”
If pH is < pI, protein is X charged
+
If pH is > pI, protein is X charged
negatively
CON- the C is-
electron deficient because of resonance. Wants to bind!
CONH has a half life of
1200 YEARS
aspartic acid and glutamate are X after being deprotonated
negative
tyrosine is X after being deprotonated
negative