2.3 Purifying DNA Experiment

Question 1

1. Break up cells using a …

Answer 1

Blender

Question 2

2. Make solution what should it contain?

Answer 2

Detergent
Salt
Distilled water

Question 3

3. What do you add to the detergent solution in a beaker?

Answer 3

The broken up cells

Question 4

4. Now you incubate the solution, at what temperature and how long for?

Answer 4

60 degrees

15 minutes

Question 5

What does the detergent in the mix do?

Answer 5

Breaks down cell membranes

Question 6

What does the salt in the solution do?

Answer 6

Binds to DNA causing it to clump

Question 7

What does the temperature of the water bath do?

Answer 7

Stops enzymes working and breaking down the DNA

Question 8

5. Now what do you put the mixture in? And what do you then do?

Answer 8

Ice bath to cool

Once cooled filter the mixture and transfer sample into clean boiling tube

Question 9

6. Next add protease enzymes, what do they do?

Answer 9

Break down proteins in mixture

Question 10

6. Also add RNAse enzyme, what will this do?

Answer 10

Break down any RNA in mixture

Question 11

7. Now what do you dribble down the side of the tube and what does it do?

Answer 11

Cold ethanol

Forms layer on top of mixture

Question 12

8. Leave for a few minutes and what happens?

Answer 12

DNA forms white precipitate