2.4 Catalase Enzyme Experiment

Question 1

What is the IV in this experiment?

Answer 1

Temperature

Question 2

What is the DV in this experiment?

Answer 2

Volume of O2

Question 3

What are other variables that need to be controlled?

Answer 3

PH
Substrate concentration
Enzyme concentration

Question 4

1. Set up boiling tubes with same volume and concentration of what?

Answer 4

Hydrogen peroxide

Question 5

1. How do you keep PH constant, what does this do?

Answer 5

Adding buffer solution

Resists changes in PH when small amounts of acids and alkalis are added

Question 6

2. What apparatus is set up to measure volume of O2 production?

Answer 6

Delivery tube and upside down measuring cylinder

Question 7

3. Each boiling tube containing hydrogen peroxide is put where?

Answer 7

Water bath

Question 8

3. What is different about each water bath?

Answer 8

Each set to different temperature

Question 9

3. What else is put in a water bath other than hydrogen peroxide solution?

Answer 9

Tube containing catalase

Question 10

3. How long do you wait for each enzyme to reach set temperature before removing from water bath?

Answer 10

5 minutes

Question 11

4. Then use a pipette to add what?

Answer 11

The same volume and concentration of catalase to each tube

Question 12

5. The record O2 production in the …

Answer 12

First minute reaction

Question 13

6. How many times do you repeat the experiment?

Answer 13

3 times at each temperature

Question 14

6. What do you then use the results to calculate?

Answer 14

Mean O2 production at each temperature

Question 15

7. You can then calculate mean rate of reaction at each temperature, what is the calculation for this?

Answer 15

Volume of O2 produced ÷ time taken (cm3s-1)