benedicts solution/ reducing sugars test

Question 1

hypothesis for benedicts solution reducing sugars test

Answer 1

if we compare the colour of test tubes containing food samples and benedicts solution to standard solutions of glucose, 7up will have a high glucose concentration, oil will have normal and milk will have a low sugar concentration

Question 2

independent varaiable for benedicts solution reducing sugars test

Answer 2

range of food samples

Question 3

dependent variable for benedicts solution reducing sugars test

Answer 3

colour of benedicts solution

Question 4

controlled (fixed) variable for benedicts solution reducing sugars test

Answer 4

same volume of foods

Question 5

control (comparison) for benedicts solution reducing sugars test

Answer 5

water

Question 6

equipment and materials for benedicts solution reducing sugars test

Answer 6

test tubes, test tube rack, 10% glucose solution, distilled water, syringe, droppers, benedicts solution, water bath, food samples

Question 7

accuracy and prescision consideration for benedicts solution reducing sugars test

Answer 7

use fresh pipettes to avoid cross-contamination

Question 8

step 1 for benedicts solution reducing sugars test (preparing a serial dilution)

Answer 8

using a pipette add 10cm3 10% glucose solution to one test tube

Question 9

step 2 for benedicts solution reducing sugars test (preparing a serial dilution)

Answer 9

using a clean pipette add 9cm3 of water to the rest of the test tubes

Question 10

step 3 for benedicts solution reducing sugars test (preparing a serial dilution)

Answer 10

using a clean pipette add 1ml of solution from the first test tube to the next and so on, using a fresh pipette each time and swirling

Question 11

step 4 for benedicts solution reducing sugars test (preparing a serial dilution)

Answer 11

remove 1ml from the last test tube

Question 12

step 5 for benedicts solution reducing sugars test (preparing a serial dilution)

Answer 12

control = test tube with 9mls of water

Question 13

step 6 for benedicts solution reducing sugars test (preparing a serial dilution)

Answer 13

add 1ml of benedicts solution to each test tube, swirl and place in hot water bath at 80 degrees for 5mins. record colour changes

Question 14

step 1 for benedicts solution reducing sugars test (testing concentration of food samples)

Answer 14

label different test tubes with different food samples

Question 15

step 2 for benedicts solution reducing sugars test (testing concentration of food samples)

Answer 15

add 9ml of each food sample to the corresponding test tube

Question 16

step 3 for benedicts solution reducing sugars test (testing concentration of food samples)

Answer 16

add 1ml of benedicts solution to each and swirl

Question 17

step 4 for benedicts solution reducing sugars test (testing concentration of food samples)

Answer 17

place in hot water bath at 80 degrees for 5 mins.

Question 18

step 5 for benedicts solution reducing sugars test (testing concentration of food samples)

Answer 18

compare test tubes to test tubes from part 1 , preparing a serial dilution , and record concentration of glucose

Question 19

0% concentration of glucose colour

Answer 19

blue, water, oil, 7up0

Question 20

0.01% concentration of glucose colour

Answer 20

green. ng

Question 21

0.1% concentration of glucose colour

Answer 21

yellow, ng

Question 22

1% concentration of glucose colour

Answer 22

brown, bread, milk

Question 23

10% concentration of glucose colour

Answer 23

orange, 7up

Question 24

15% concentration of glucose colour

Answer 24

brick red, 7up

Question 25

reproducibilty etc

Answer 25

used clean pipettes each time to avoid cross contamination, used known standard solutions of glucose, carried out step by step method