Bio - 5.1

Question 1

enzymes

Answer 1

• binded to one particular substrate
• break down substances bigger to smaller
• function as a biological catalysts

Question 2

properties of enzymes

Answer 2

• all enzymes are protein
• catalysts
• specific
• work best at a particular temp or pH
• reusable

Question 3

active site

Answer 3

• region on an enzyme that binds to a substrate during a reaction

Question 4

amylase

Answer 4

substrate = starch
products = maltose

Question 5

maltase

Answer 5

substrate = maltose
product = glucose

Question 6

protease

Answer 6

substrate = protein
product = amino acids

Question 7

lipase

Answer 7

substrate = fats
products = fatty acid + glycerol

Question 8

cellulase

Answer 8

substrate = cellulose
products = glucose

Question 9

lock and key mechanism

Answer 9

1. substrate will bind into the active site
2. enzyme substrate complex
3. enzyme will break down larger molecule to smaller molecule

Question 10

catalysts

Answer 10

• substances that speeds up the rate of chemical reaction

Question 11

pH and enzyme activity

Answer 11

• rate of reaction depends on optimum pH
• change is pH (too high/low) alters the shape of active site of enzymes, causing them to denature

Question 12

temperature and enzyme activity

Answer 12

• temperature rises => enzyme and substrate molecules gain more kinetic energy => move faster
• rate of reaction will increase until optimum temperature
• temp is too high the enzyme will denature

Question 13

benedicts test

Answer 13

REDUCING SUGAR
- blue = no sugar
- red = sugar

Question 14

biuret test

Answer 14

PROTEIN
- purple: protein is present
- blue: protein isn’t present

Question 15

iodine test

Answer 15

STARCH
- brown: no starch
- black: starch

Question 16

DCPIP test

Answer 16

VITAMIN C
colorless = present
blue = not present