A patient presents with UTI symptoms. Urine sample plated.
Gram stain shows: Gram-negative rods.
Growth on MacConkey pink colonies
EMB agar metallic green
Indole test positive
Final ID?
- Rules out gram-positive organisms.
- Lactose fermentation.
- Strong lactose fermenter
- Tryptophan —> indole
Escherichia coli
Wound infection culture.
Gram-positive cocci in clusters. What arrangement plane?
Growth on MSA: Yellow colonies. What happened?
Blood agar: Clear zone. Type of hemolysis?
Coagulase test: Positive. What does this mean?
Multiple/random planes (clusters).
Mannitol fermentation → acid → yellow.
Beta hemolysis.
Plasma clot formation.
Staphylococcus aureus
Patient with sore throat.
What division pattern?
Hemolysis type?
Catalase negative. What does this rule out?
Bacitracin sensitivity Significance?
One plane (chains).
Beta hemolysis.
Rules out Staphylococcus.
Group A strep indicator.
Streptococcus pyogenes
