Which part of the microscope gathers and focuses light on the specimen?
Condenser lens
Approximate size of a bacterial cell?
0.5-2.0 micrometers (um)
What type of bacteria is Treponema pallidum?
Spirochete (flexible spiral bacterium)
How do you obtain the greatest resolution in light microscopy?
Use shortest wavelength of light + highest numerical aperture objective
What are fimbriae?
Short, hairlike bristles used for adhesion
What are pili primarily used for?
Conjugation (DNA transfer)
When is immersion oil used?
Only with 100x oil immersion objective
Difference between wet mount and hanging drop?
Hanging drop allows longer observation of motility (less drying)
What is resolving power?
Ability to distinguish two close objects as separate
If you could increase only one for better detail - magnification or resolution?
Resolution
Resolving power formula?
Wavelength / (2 x Numerical Aperture)
NA = n x sin(0)
0.008 mm equals how many micrometers?
8 um
Unit used to measure bacteria?
Micrometer (um)
What is binary fission?
Asexual reproduction
Purpose of Koch’s Postulates?
To establish a causal relationship between a microorganism and a disease
Domain containing organisms in extreme environments?
Archaea
First word in a scientific name?
Genus
Second word in binomial nomenclature?
Species
Bacteria with clusters of flagella?
Lophotrichous
Endospores are stained with what?
Malachite green
Arrangement of Staphylococcus aureus?
Clusters
Spirilla are described as?
Stiff, spiral-shaped bacteria
Brownian movement is…
Random and passive (not true motility)
What does safranin do in Gram staining?
Counterstain (stains Gram-negative pink)
