What is a chromosome?
A long, tightly packed strand of DNA.
What is DNA?
The molecule that carries genetic instructions.
What is a gene?
A section of DNA that codes for a protein.
What does each gene code for?
A protein
What do proteins control?
Traits.
What is a trait?
A physical or biological characteristic like eye color or blood type.
What is transcription?
Making RNA from a DNA template.
What enzyme performs transcription?
RNA polymerase.
Where does transcription occur in eukaryotes?
In the nucleus.
Where does transcription occur in prokaryotes?
In the cytoplasm.
In what direction is DNA read during transcription?
3′ → 5′.
In what direction is mRNA built?
5′ → 3′.
What is the template strand?
The DNA strand RNA polymerase reads.
What is the coding strand?
The DNA strand that matches mRNA except T is replaced with U.
What is a promoter?
A DNA region where RNA polymerase binds to start transcription.
What does upstream mean?
DNA before the transcription start site.
What does downstream mean?
DNA after the transcription start site.
What is the –35 region?
A promoter region 35 bases before the start site.
What is the –10 region?
A promoter region 10 bases before the start site.
What are the three phases of transcription?
Initiation, elongation, and termination.
What happens during initiation?
RNA polymerase binds the promoter and starts RNA synthesis.
What is the RNA polymerase holoenzyme?
Core enzyme plus sigma factor.
What does the sigma factor do?
Recognizes the promoter and separates DNA.
What happens during elongation?
RNA polymerase builds the RNA strand.