Controls

Question 1

Purpose of Controls in IHC: (2)

Answer 1

1. To test for specificity of antibodies involved
2. To prevent misinterpretation due to false (+) and false (-) results

Question 2

Control where the tissue section on slide containing the antigen is being detected

Answer 2

Positive Control

Question 3

How to prepare negative control? (2)

Answer 3

1. Omit the primary antibody from staining
   schedule
2. Replace the primary antibody by an Ig that is
   directed against an unrelated antigen

Question 4

Control that contains the target antigen not only in the tissue elements but also in adjacent normal tissue elements

Answer 4

Internal Tissue Control/ Built-in Control