Processed Specimen

Question 1

This is to be done only for paraffin embedded &
formalin fixed tissues

Answer 1

Pre-treatment blocking

Question 2

Pre-treatment blocking should not be done on which type of specimen?

Answer 2

Frozen Sections

Question 3

Methods in Pre-treatment blocking: (2)

Answer 3

1. Peroxidase Block
2. Protein Block

Question 4

Purpose of Peroxidase block and its reagent.

Answer 4

a. To block endogenous peroxidases

b. Uses 3% H2O2

Question 5

Purpose of Protein block and its reagent

Answer 5

a. Blocks all non-specific sites & reduces
background (more preferred)

b. Uses 10% Normal Serum