Enzyme classification

Question 1

Proof of amylase?

Answer 1

Lugols solution

Question 2

Proof of peroxidase in blood?

Answer 2

catalase cleaves h2o2 to water and oxygen
peroxidase removes oxygen from h2o2

blue indigo carmine is oxidezed—colourless

Question 3

what do oxido-recutase, transferase, hydrolase, lyase, isomerase, ligases do?
Units for ligases are?
This unit is equal to 1_/__

Answer 3

1. oxido-reductase -catalyse oxidation, reduction reactions (eg.
   Dehydrogenases)
2. transferase -transfer functional group from one substrate to another (eg.
   Amino transferase, kinases- Pi from one ATP to other molecules)
3. hydrolase -catalyse hydrolysis of chemical bond, breaking bond using water
   (eg. Phosphatases)
4. lysase –catalyse the breaking of chemical bond by means other that
   hyrdolysis or oxidation, thus forming new double bond
5. isomerase -catalyse isomeric rearrangements
6. ligases -catalyse joining of one molecule to another using ATP
   units = katal
   1katal = 1mol/s
   katal: catalytic activity that will raise rate of reaction by 1 mole per second.