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histopath Flashcards

(40 cards)

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1
Q
  1. Enzymes used for antigen retrieval in IHC
    1) Phosphatase
    2) Trypsin
    3) Lipase
    4) Protease

A. 1&3
B. 2&4
C. 1,2 & 3
D. 1,2,3 & 4

A

B. 2&4

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2
Q
  1. Which of the following statements is incorrect?

A. pre treatment blocking should only be done for paraffin embedded tissues
B. in the preparation of negative control, omit the primary antibody from the staining schedule
C. Indirect IHC is more sensitive than direct because of belter signal amplification
D. none of these

A

D. none of these

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3
Q
  1. all of the following are methods of antigen retrieval except.

A. Microwave technique
B. incinerator healing
C. autoclave heating
D. decloaker healing
E. none

A

B. incinerator healing

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4
Q
  1. advantages of enzymatic IHC except:

A. fluorescence microscope is not required
B. hematoxylin can be used as counterstain
C. standard samples can be stored for a long period
D. none of these

A

D. none of these

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5
Q
  1. sources of polyclonal antibodies
    1) sheep
    2) Goat
    3) Rabbits
    4) mice

A. 1&3
B. 2&4
C. 1,2 &3
D. 123 &4

A

C. 1,2 &3

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6
Q
  1. compatible counterstains for DAB
    1) brilliant green
    2) Methyl violet
    3) eosin
    4) Safranin

A. 1&3
B. 2&4
C. 1,2 & 3
D. 1, 2, 3 & 4
E. none of these

A

E. none of these

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7
Q
  1. if alkaline phosphatase was used to label antibodies in IHC which of the following can be used?
    1) DAB
    2) Fast Red TR salt
    3) Amino ethyl carbazole
    4) BCIP

A. 1&3
B. 2&4
C. 1,2 & 3
D. 1,2,3 & 4
E. none of these

A

B. 2&4

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8
Q
  1. which of the following chromogens that when used will produce a bluish violet or black violet color?

A. Fast Red TR Salt
B. DAB
C. amino ethyl carbazole
D. BCIP
E. none of these

A

D. BCIP

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9
Q
  1. in IHC, what is the incubation time and temperature to link antibody with enzyme?

A. 30-60 mins 37 degC
B. 30-60 minutes at RT
C. 1-2 hrs at RT
D. none of these

A

B. 30-60 minutes at RT

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10
Q
  1. which of the following is the most commonly used enzyme label in IHC?

A. FITC
B. HRP
C. AP
D. DAB
E. none of these

A

B. HRP

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11
Q
  1. In IHC, which form of hematoxylin that when used tissue morphology are well defined?

A. Mayer’s
B. Ehrlich’s
C. Coles
D. Harris
E. all of these

A

D. Harris

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12
Q
  1. acceptable specimens for IHC except.

A. paraffin embedded
B. formalin fixed
C. frozen section
D. none of these

A

D. none of these

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13
Q
  1. which of the following is an oncofetal antigen that is very useful in differentiating mesothelioma and adenocarcinoma

A. Epithelial membrane antigen
B. CEA
C. Vimentin
D. Desmin
E. none of these

A

B. CEA

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14
Q
  1. from the choices in # 13, which is highly specific for myogenic tumors?

A. Epithelial membrane antigen
B. CEA
C. Vimentin
D. Desmin
E. none of these

A

D. Desmin

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15
Q
  1. Which of the following is the marker for choriocarcinoma?
    A. CEA
    B. PSA
    C. HCG
    D. alpha feto protein
    E. all of these
A

C. HCG

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16
Q
  1. The tumor marker that will confirm diagnosis of astrocytoma?

A. GFAP
B. PSA
C. CEA
D. actin
E. none of these

A

A. GFAP

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17
Q
  1. an epithelial tumor marker which is also positive in certain pancreatic and salivary gland cancer

A. PSA
B. CEA
C. HCG
D. desmin
D. None of these

18
Q
  1. conditions that will stan positive for vimentin

A. schwannomas
B. seminoma
C. melanoma
D. none of these
E. all of these

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A

E. all of these

19
Q
  1. which of the following is synthesized by placental syncitiotrophoblast?

A. CEA
B. PSA
C. HCG
D. alpha feto protein
E. all of these

20
Q
  1. all are intermediate filament markers except:

A. TTF-1
B. Vimentin
C. GFAP
D. Desmin
E. none of these

21
Q
  1. which of the following is more common in carcinomas of the colon and stomach?

A. CK7
B. CK20
C. EMA
D. none of these

22
Q
  1. From the choices in # 21, which can be helpful in determining tumor site?

A. CK7
B. CK20
C. EMA
D. none of these

23
Q
  1. most common form of antibody used in IHC?

A. lgA
B. lgM
C. lgG
D. IgE
E. none of these

24
Q
  1. True of IHC except:

A. allows detection of organism in cytologic preparations like FNA
B. it is basically an antigen antibody reaction
C. automation can provide greater consistency when it comes to staining
D. specimen or IHC is limited only to processed specimens like paraffin embedded tissues
E. none of these

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A

D. specimen or IHC is limited only to processed specimens like paraffin embedded tissues

25
25. uses of this chromogen can lead to production of Brick red color A. DAB B. BCIP C. AEC D. fast Red TR salt
C. AEC
26
26. cells found in cervico vaginal smear with a diameter of 15-30 u A. superficial cells B. Basal cells C. parabasal cells D. Intermediate cells
C. parabasal cells
27
27. From the choices in # 26, which cell has basophilic vacuolated cytoplasm A. superficial cells B. Basal cells C. parabasal cells D. Intermediate cells
D. Intermediate cells
28
28. in Pap's staining of non-gynecological specimens. Which form of hematoxylin is used in nuclear stain? A. Mayer's B. Harris C. Ehrlich's D. Gill's E. none of these
B. Harris
29
29. which of the following are used as counterstains in PAP's staining? 1) OG50 2) EA50 3) EA 60 4) 0G6 A. 1&3 B. 2&4 C. 1,2&3 D. 1,2,3 & 4 E. none of these
B. 2&4
30
30. which of the following cells in cervico vaginal smear is shed in response to ovarian hormone? A. navicular cells B. pregnancy cells C. endometrial cells D. endocervical cells
C. endometrial cells
31
31. These are boat shaped cells with a tendency to fold or curl on edges. Use choices in # 30 A. navicular cells B. pregnancy cells C. endometrial cells D. endocervical cells
A. navicular cells
32
32. From the choices in # 30, which may assume a "honeycomb* appearance? A. navicular cells B. pregnancy cells C. endometrial cells D. endocervical cells
D. endocervical cells
33
33. The presence of these cells suggest a combined estrogen-progesterone effect? Use choices in # 30 A. navicular cells B. pregnancy cells C. endometrial cells D. endocervical cells
A. navicular cells
34
34. Predominance of which of the following cells is noted following childbirth? Use choices in #30 A. navicular cells B. pregnancy cells C. endometrial cells D. endocervical cells
E. none of these -Parabasal
35
35. These are polygonal squamous cells measuring 45-50 um In diameter. Use choices In # 30 A. navicular cells B. pregnancy cells C. endometrial cells D. endocervical cells
E. none of these -Superficial
36
36. in Pap's staining of gynecological specimens. Which form of hematoxylin is used as nuclear stain? A. Mayer's B. Harris C. Ehrlich's D. Gill's E. none of these
B. Harris
37
37 In PAP's staining of the following will that when used will stain the cytoplasm of other cells including non-keratinized squamous cells A. OG8 B. Light Green SF C. Bismarck Brown Y D. EA 36 E. none of these
B. Light Green SF
38
38. In Cytochemistry, which can be used to stain glycogen? A. Perl's Prussian Blue B. Grocott Methenamine Silver C. Periodic Acid Schiff D. Alcian Blue E. none of these
C. Periodic Acid Schiff
39
39. From the choices in # 38, which is considered to be the staining method of choice in Exfoliative cytology. A. Perl's Prussian Blue B. Grocott Methenamine Silver C. Periodic Acid Schiff D. Alcian Blue E. none of these
E. none of these -Pap's
40
40. All of the following statements are correct except: A. FN4 involves study of cellular samples obtained from organs that DO NOT SHED CELLS spontaneously B. Cytospin preparation is by far the best method to collect cells from body fluids C. absence of alveolar macrophage suggest that the collected specimen is merely saliva and not sputum D. prevention of jelly clots from collected body fluid can be prevented using 300 units of heparin E. None of these
E. None of these
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