Lab Practical

Question 1

As magnification increases, depth of focus:

Answer 1

Decreases

Question 2

Ocular Lens

Answer 2

Lens where the eye is placed on a microscope

Question 3

Scanning magnification
Low power magnification
High power magnification

Answer 3

4
10
40

Question 4

Bradford Assay

Answer 4

Tests for protein presence: Red “bradford” solution mixed with a sample, and if it’s positive for protein, it will turn blue. Can be checked for absorbance value using spectrophotometer, so technically quantitative.

Question 5

Iodine Test

Answer 5

A sample is tested for starch presence. Sample is mixed with iodine: if product has no starch, product remains as the yellow-brown color of iodine. If starch is present, product turns bluish black (due to the coiled structure of starch). QUALITATIVE.

Question 6

Point of Saturation

Answer 6

In a reaction catalyzed by an enzyme, the point where there’s so much substrate that adding more won’t do anything.

Question 7

DOPA

Answer 7

Serves as substrate

Question 8

Phenolase

Answer 8

Enzyme that comes from potatoes

Question 9

Emulsion Test

Answer 9

A sample is tested for presence of lipids. dissolved in ethanol, then water is added. If lipids are present, a cloudy white emulsion forms. QUALITATIVE

Question 10

Calvin Cycle

Answer 10

Dark Reactions of photosynthesis

Question 11

Independent Assortment

Answer 11

During metaphase I of meiosis I, Tetrads line up along the metaphase plate. The parental or maternal chromosome has a 50% chance of ending up in either one of the daughter cells that result from meiosis I.
i.e. it’s not all maternal chromosomes going to one cell and all paternal going to the other: there’s a mix.

Question 12

Benedict’s Test

Answer 12

Tests a sample for presence of “reducing sugars”. Mix sample with blue benedict’s reagent, then boil the solution. turns brick red if positive. Qualitative.