Lecture 10

Question 1

How does sorting of proteins occur at TGN

Answer 1

• Storage / transport via clathrin coated vesicles
• Proteins tagged in Cis Golgi
• Phosphorylated mannose residues
• Rcognized or captures: Mannose 6 Phosphate receptors

Question 2

Role of Adaptin

Answer 2

• Create a bridge of adaptin
• induce Curvature
• Cargo selection and Cargo receptor

Question 3

what does tethering depend on

Answer 3

Rab protein
tethering protein

Question 4

Rab proteins

Answer 4

small G protein family
GTP bound = active
GDP bound = inactive

Question 5

SNARE prtoeins

Answer 5

For docking
Brings it close enough for fusion
v snare = vesicle
t snare = target

Question 6

2 types of t snares

Answer 6

• Syntaxin
• 2 SNAP 25

Question 7

V SNARE proteins

Answer 7

Synaptobrevin

Question 8

How many t snares and v snares interact

Answer 8

• 4 Proteins

Question 9

Luminal part

Answer 9

Extracellular surface of plasma membrane

Question 10

Cytosolic part

Answer 10

Intracellular surface

Question 11

Two types of Endocytosis

Answer 11

1. Bulk phase
2. Receptor mediated endocytosis

Question 12

main destination of endocytic pathway

Answer 12

lysosomes

Question 13

Lysosomes

Answer 13

is acidic
ATP and H+

Question 14

Peroxisomes

Answer 14

• Small membranous organelle
• Carrying out metabolic functions
• Transport proteins across membranes
• Synthesis on free ribosomes
• Imported posttranslationally