microbio lab 2 Flashcards

(17 cards)

1
Q

what does the gelatin test test for

A

gelatinase activity

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2
Q

grlatin test results

A

pos-liquidificaiton
neg-solid

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3
Q

what does the sugar fermentation test teast for

A

glucos, lactos, and manitol

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4
Q

what is used in the fermentation test

A

Durham tube to collect gas
Phenol red as ph indicator

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5
Q

fermentaiton test results

A

red - alkaline - neg for sugar
yellow - pos for sugar fermentation - acid+gas or no gas

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6
Q

what does Voges-Prosauer test for

A

acetone precursors

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7
Q

Voges Prosauer test results

A

red-pos
yellow-neg

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8
Q

what does Methyl red test for

A

mixed acid fermentation

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9
Q

Methyl red tes results

A

red-pos-acidic
yellow-neg-alkaline

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10
Q

nitrate reduction results

A

prink strip-no bubbles-pos for nitrate reduction
none pink strip-bubbles-pos for nitrate reduction
none pink strip-no bubble- neg for nitrate reduction

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11
Q

citrate test results

A

green-neg
blue-pos-using sodium citrate as only source of carbon

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12
Q

casine hydrolysis results

A

for cansinase

pos - clear halo around growth
neg - only growth

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13
Q

Fat Hydrolysis results

A

for lipase

pos - dark halo around growth
neg - none/growth

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14
Q

starch hydrolysis results

A

for amylase

pos - light halo around growth
neg - dark background around growth

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15
Q

trypton broth results

A

for tryptophanase

pos - pink ring
neg - yellow ring

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16
Q

vern hydrolysis results

A

for urea

pos - pink
neg - light orange