Microscopy

Question 1

What are the qualities of a light microscope?

Answer 1

Poor resolution due to the long wavelength of light.
Living samples can be examined and a colour image is obtained.

Question 2

What are the qualities of a Transmission electron microscope?

Answer 2

High magnification and resolution.
Electrons pass through the specimen to create a 2D, black and white image.

Question 3

What are the qualities of a Scanning electron microscope?

Answer 3

High magnification and resolution.
Electrons bounce off the surface of the specimen to create a 3D, black and white image.

Question 4

What are the qualities of a Laser scanning confocal microscope?

Answer 4

High resolution and 3D imaging.
Laser light is used to create image.

Question 5

What is resolution?

Answer 5

minimum distance between two objects in which they can still be viewed as separate

Question 6

What is magnification?

Answer 6

refers to how many times larger the image is compared to the object.

Question 7

What are the steps for the process of calibrating a microscope

Answer 7

Step 1- Line up the stage micrometer and eyepiece graticule whilst looking through the eyepiece.
Step 2- Count how many divisions on the eyepiece graticule fit into one division on the micrometer scale.
Step 3- Each division on the micrometer is 10 μm, so this can be used to calculate what one division on the eyepiece graticule is at the current magnification.

Question 8

What is the calculation for magnification?

Answer 8

size of image/ actual size of object

Question 9

What are the four types of sample preparations (slides)

Answer 9

Dry mount
Wet mount
Squash slide
Smear slide

Question 10

What is a dry mount?

Answer 10

thin slices or whole organism viewed, just with the coverslip on top

Question 11

What is a wet mount?

Answer 11

viewing the specimen with water added/stain then coverslip added.

Question 12

What is a squash slide

Answer 12

Wet mounts of which are pushed down to create a thinner slice.

Question 13

What is a smear slide?

Answer 13

Created by adding a drop of the sample of on side then using the edge of another slide to smear it across.

Question 14

What is differential staining?

Answer 14

Using different chemical stains like crystal violet, methylene blue or Congo red to stain different parts of a cell in different colors to increase visibility.

Question 15

Example rules for scientific drawing?

Answer 15

Pencil
Title
State magnification
Label key features
No shading
No colour
Straight lines

Question 16

What variables need to be controlled during an experiment?

Answer 16

Temp
pH
Volumes
Concentrations
Consistency of organism
Time of day
Enough values

Question 17

Improvements to color change studies.

Answer 17

Use a colorimeter, measure time taken to reach an absorbance value

Question 18

Improvements to pH studies.

Answer 18

Use a pH probe and measure time taken to reach set pH

Question 19

DIfference between descibe and explain questions

Answer 19

Explain need stats and figures, desbine does not.

Question 20

Order of microscope resoloution and magnification

Answer 20

Light
Laser
Scanning
Transmission