Difference between prokaryotic and eukaryotic
Prokaryotic (“before the nucleus”) cells - form the bodies of bacteria and archaea and is the simplest forms of life.
Eurkaryotic, unicellular
to multicellular organisms
* Membrane-bounded nucleus
* Sexual reproduction
* Phenotypes and nutrition are diverse
* Each kingdom has specializations * Flagella, if present, have
a 9 + 2 organization
Bacteria
* Prokaryotic, unicellular organisms
* Lack a membrane-bounded nucleus
* Reproduce asexually
* Heterotrophic by absorption * Autotrophic by chemosynthesis
or by photosynthesis * Move by flagella
archea
Archaea
* Prokaryotic, unicellular organisms
* Lack a membrane-bounded nucleus
* Reproduce asexually * Many are autotrophic by chemosynthesis; some are
heterotrophic by absorption
* Unique rRNA base sequence * Distinctive plasma membrane
and cell wall chemistry
Chromosomes
DNA coiled together attached to histoine protein
The complete DNA–protein structure that carries genetic information
Exists throughout the cell cycle
Can be unduplicated (one chromatid) or duplicated (two sister chromatids)
Counted as one chromosome whether it has one or two chromatids
Found in both body cells and sex cells
Chromatid
One of the two identical copies of a chromosome after DNA replication
Exists only after the S phase of interphase
Sister chromatids are joined at the centromere
Each chromatid becomes an individual chromosome when they separate during cell division
Biggest difference between eukaryotic and prokaryotic
Eu, have nucleus with nuclear envelope, bigger more complex, often multicellular, always have nucleus bound organelles, dna is learn, ribosomes large, cell walls made out of cellulose, cell division by mitosis or meiosis’s
Pro: always unicellar, no nucleus, dna is circular and free floating, ribosomes are small, cell wall made of peptide glycol cell division is binary fission
Parts. Of a nucleotide
Phosphate group, sugar group, one of four types of nitrogen bases, adenine, thymine, cytosine, guanine
Hydrogen bonds
Hydrogen bonds hold complementary base pairs together in DNA
Prokaryotic cell cycle
The prokaryotic chromosome, a circular DNA double helix, is attached to the plasma membrane
at one point. * The DNA replicates and the resulting two chromosomes attach to the plasma membrane at
nearby points. 3 New plasma membrane is added between the attachment points, pushing the two
chromosomes farther apart. 4 The plasma membrane grows inward at the
middle of the cell.
5. The parent cell divides into two daughter Cells
Difference between prokaryotic and eukaryotic chromosomes
Eukaryotic chromosomes differ from prokaryotic chromosomes:
●Chromosomes separated from cytoplasm by a membrane-bound nucleus;
●Always have multiple chromosomes and occurs in pairs;
His tone and chromatin organizaton ion
The DNA molecule winds around histones to form a
bead-like structure.
T
30 nm B The bead-like structure forms a tightly packed array to produce strands
of chromatin.
T
300 nm Chromatin fibres form loops, which are attached to a supporting protein
scaffold.
T
700 nm
centromere
上
T
1
1400 nm
condensed chromosome pair Figure 16.2 The levels
of organization of genetic The scaffold folds further to condense the genetic material into chromosomes, duplicate during replication, forming pairs of identifical chromosomes joined
by a centromere.
AUTOSOME and somatic
Autosome are diploid (2n) and found in Somatic cells; Somatic cells have 46 chromosomes (autosomes).
Sex chromosomes
Sex chromosomes are haploid (n) and found in Sex cells; Sex chromosomes (X and Y) determine sex of an ind.:
-Include egg and sperm;
-contain half no. of chromosomes of a diploid cell
Differences between autosomes and sex chromosomes
Differences between Autosomes and Sex chromosomes:
➢Vary in length;
➢Location of their centromere;
➢Staining properties.
genes
Segments of DNA are called genes (units of inheritance).
haploid vs diploid
Autosome are diploid (2n) and found in
Somatic cells; Somatic cells have 46
chromosomes (autosomes).
Sex chromosomes are haploid (n) and found
in Sex cells; Sex chromosomes (X and Y) determine
sex of an ind.:
- Include egg and sperm;
- contain half no. of chromosomes of a diploid
cell;
- When sperm fertilizes an egg, fusion of two
haploid cells produces a diploid cell
SRY gene/ difference between X and Y chromosomes
X chromosome does not contain SRY gene, which is the male
determining gene; Y chromosome has SRY gene;
X chromosome is larger in size and contains a higher no. of genes
compared to Y chromosome;
Y chromosome is smaller in size and contains only a few no. of genes
CHROMATRIN DEFINE
Chromatin is a complex of DNA, RNA, and proteins—primarily histones—found in eukaryotic cell nuclei, functioning to package long DNA molecules into compact structures. It is composed of “nucleosomes” (DNA wrapped around histone octamers) and is produced by the condensation of DNA with histone proteins during interphas
Karyotype
no. and visual appearance of chromosomes in the cell
nuclei of an org.
Chromatin
Definition:
Chromatin is the DNA–protein complex found in the nucleus of eukaryotic cells that makes up chromosomes.
Key points:
Consists of DNA + histone proteins + non-histone proteins
Exists as euchromatin (light, active) and heterochromatin (dark, inactive)
Condenses to form chromosomes during cell division
Involved in gene regulation and DNA packaging
Histone
Definition:
Histones are basic (positively charged) proteins that DNA wraps around to form chromatin.
Key points:
Rich in lysine and arginine
Types include H2A, H2B, H3, H4 (core histones) and H1 (linker histone)
Help in DNA packaging and structural support
Play a role in gene expression regulation via chemical modifications
genes vs alleles
Genes –section of chromosome that codes for a specific protein
Determines a specific trait (hair color, eye color, ear shape etc.
locus (plural, loci) is the location of a gene on a chromosome.
Paired on homologous chromosomes
-Alleles – Different forms/version of genes found at the same
gene locus;
For each gene there are two alleles (one on each
homologous chromosome) which may be same or
different:
homo vs heterozygous
If both homologous chromosomes carry the same alleles
for that gene, the org. is Homozygous
- If both homologous chromosomes carry diff. alleles for
that gene, the org. is Heterozygous.
homologous chromosomes
Homologous chromosomes possess the same genes at the same loci (locations) because they are matched pairs—one inherited from each parent—but they do not necessarily have the same alleles. While they share the same structure and gene order, the specific alleles (versions of a gene) at a particular locus may be identical or differen
sister chromatids
sister chromatids of duplicaated chromosomes have same alleles for each gene
Prior to cell division, the DNA is replicated
At the end of DNA replication, a duplicated
chromosome consists of two identical DNA double
helices, called sister chromatids, which are
attached to each other at the centromere
= Sister chromatids are two identical copies of the same chromosome that are joined together.
They form when DNA is copied
They are attached at the centromere
They carry the same genes
what happens to sister chromatids during mitotic cell divison?
During mitotic cell division, the two sister chromatids
separate, each becoming an independent
chromosome that is delivered to one of the two
daughter cells
Replication
and
Compaction of
Chromosomes
into Pairs of
Sister
Chromatids process
1) each chromosomes replicates before mitosis
2)at the start of mitsoisd, the chromosomes become compact
sequence of events from one cell division to the next
Process by which genetic material is duplicated and passed from parent cell to daughter cell;
- Each daughter cell receives a complete set of hereditary info. and half its cytoplasm from parent
cell Hereditary information usu. Identical to parent cell.
Process: The cell cycle (mitosis + cytokinesis)
“Genetic material is duplicated”
→ Interphase (S phase) — DNA is copied
“Passed from parent cell to daughter cell”
→ Mitosis — chromosomes are separated
“Each daughter cell receives a complete set of hereditary information”
→ Mitosis
“Half its cytoplasm from the parent cell”
→ Cytokinesis
“Hereditary information usually identical to parent cell”
→ Mitosis (produces identical cells)
stages of the cell cycle
Consists of two (2) main stages: Interphase and Mitotic Cell Division.
1. Growth Stage (Interphase) - acquisition of nutrients, growth, and chromosome duplication.
Cell grows in size, replicates DNA, and differentiates. Involves three (3) phases: G1, S and G2.
1. Growth 1 (G1) Phase: acquisition of nutrients and growth
2. Synthesis (S) Phase: DNA synthesis, every chromosome is replicated
3. Gap 2 or Growth 2 Phase (G2): completion of cell growth and preparation for division