Module 4, Cell division Flashcards

(151 cards)

1
Q

Difference between prokaryotic and eukaryotic

A

Prokaryotic (“before the nucleus”) cells - form the bodies of bacteria and archaea and is the simplest forms of life.

Eurkaryotic, unicellular
to multicellular organisms
* Membrane-bounded nucleus
* Sexual reproduction
* Phenotypes and nutrition are diverse
* Each kingdom has specializations * Flagella, if present, have
a 9 + 2 organization

Bacteria
* Prokaryotic, unicellular organisms
* Lack a membrane-bounded nucleus
* Reproduce asexually
* Heterotrophic by absorption * Autotrophic by chemosynthesis
or by photosynthesis * Move by flagella

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2
Q

archea

A

Archaea
* Prokaryotic, unicellular organisms
* Lack a membrane-bounded nucleus
* Reproduce asexually * Many are autotrophic by chemosynthesis; some are
heterotrophic by absorption
* Unique rRNA base sequence * Distinctive plasma membrane
and cell wall chemistry

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3
Q

Chromosomes

A

DNA coiled together attached to histoine protein

The complete DNA–protein structure that carries genetic information

Exists throughout the cell cycle

Can be unduplicated (one chromatid) or duplicated (two sister chromatids)

Counted as one chromosome whether it has one or two chromatids

Found in both body cells and sex cells

Chromatid

One of the two identical copies of a chromosome after DNA replication

Exists only after the S phase of interphase

Sister chromatids are joined at the centromere

Each chromatid becomes an individual chromosome when they separate during cell division

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4
Q

Biggest difference between eukaryotic and prokaryotic

A

Eu, have nucleus with nuclear envelope, bigger more complex, often multicellular, always have nucleus bound organelles, dna is learn, ribosomes large, cell walls made out of cellulose, cell division by mitosis or meiosis’s

Pro: always unicellar, no nucleus, dna is circular and free floating, ribosomes are small, cell wall made of peptide glycol cell division is binary fission

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5
Q

Parts. Of a nucleotide

A

Phosphate group, sugar group, one of four types of nitrogen bases, adenine, thymine, cytosine, guanine

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6
Q

Hydrogen bonds

A

Hydrogen bonds hold complementary base pairs together in DNA

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7
Q

Prokaryotic cell cycle

A

The prokaryotic chromosome, a circular DNA double helix, is attached to the plasma membrane
at one point. * The DNA replicates and the resulting two chromosomes attach to the plasma membrane at
nearby points. 3 New plasma membrane is added between the attachment points, pushing the two
chromosomes farther apart. 4 The plasma membrane grows inward at the
middle of the cell.
5. The parent cell divides into two daughter Cells

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8
Q

Difference between prokaryotic and eukaryotic chromosomes

A

Eukaryotic chromosomes differ from prokaryotic chromosomes:
●Chromosomes separated from cytoplasm by a membrane-bound nucleus;
●Always have multiple chromosomes and occurs in pairs;

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9
Q

His tone and chromatin organizaton ion

A

The DNA molecule winds around histones to form a
bead-like structure.
T
30 nm B The bead-like structure forms a tightly packed array to produce strands
of chromatin.
T
300 nm Chromatin fibres form loops, which are attached to a supporting protein
scaffold.
T
700 nm
centromere

T
1
1400 nm
condensed chromosome pair Figure 16.2 The levels
of organization of genetic The scaffold folds further to condense the genetic material into chromosomes, duplicate during replication, forming pairs of identifical chromosomes joined
by a centromere.

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10
Q

AUTOSOME and somatic

A

Autosome are diploid (2n) and found in Somatic cells; Somatic cells have 46 chromosomes (autosomes).

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11
Q

Sex chromosomes

A

Sex chromosomes are haploid (n) and found in Sex cells; Sex chromosomes (X and Y) determine sex of an ind.:
-Include egg and sperm;
-contain half no. of chromosomes of a diploid cell

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12
Q

Differences between autosomes and sex chromosomes

A

Differences between Autosomes and Sex chromosomes:
➢Vary in length;
➢Location of their centromere;
➢Staining properties.

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13
Q

genes

A

Segments of DNA are called genes (units of inheritance).

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14
Q

haploid vs diploid

A

Autosome are diploid (2n) and found in
Somatic cells; Somatic cells have 46
chromosomes (autosomes).
Sex chromosomes are haploid (n) and found
in Sex cells; Sex chromosomes (X and Y) determine
sex of an ind.:
- Include egg and sperm;
- contain half no. of chromosomes of a diploid
cell;
- When sperm fertilizes an egg, fusion of two
haploid cells produces a diploid cell

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15
Q

SRY gene/ difference between X and Y chromosomes

A

X chromosome does not contain SRY gene, which is the male
determining gene; Y chromosome has SRY gene;
 X chromosome is larger in size and contains a higher no. of genes
compared to Y chromosome;
 Y chromosome is smaller in size and contains only a few no. of genes

CHROMATRIN DEFINE
Chromatin is a complex of DNA, RNA, and proteins—primarily histones—found in eukaryotic cell nuclei, functioning to package long DNA molecules into compact structures. It is composed of “nucleosomes” (DNA wrapped around histone octamers) and is produced by the condensation of DNA with histone proteins during interphas

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16
Q

Karyotype

A

no. and visual appearance of chromosomes in the cell
nuclei of an org.

Chromatin

Definition:

Chromatin is the DNA–protein complex found in the nucleus of eukaryotic cells that makes up chromosomes.

Key points:

Consists of DNA + histone proteins + non-histone proteins

Exists as euchromatin (light, active) and heterochromatin (dark, inactive)

Condenses to form chromosomes during cell division

Involved in gene regulation and DNA packaging

Histone

Definition:

Histones are basic (positively charged) proteins that DNA wraps around to form chromatin.

Key points:

Rich in lysine and arginine

Types include H2A, H2B, H3, H4 (core histones) and H1 (linker histone)

Help in DNA packaging and structural support

Play a role in gene expression regulation via chemical modifications

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17
Q

genes vs alleles

A

Genes –section of chromosome that codes for a specific protein
 Determines a specific trait (hair color, eye color, ear shape etc.
 locus (plural, loci) is the location of a gene on a chromosome.
 Paired on homologous chromosomes
-Alleles – Different forms/version of genes found at the same
gene locus;
 For each gene there are two alleles (one on each
homologous chromosome) which may be same or
different:

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18
Q

homo vs heterozygous

A

If both homologous chromosomes carry the same alleles
for that gene, the org. is Homozygous
- If both homologous chromosomes carry diff. alleles for
that gene, the org. is Heterozygous.

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19
Q

homologous chromosomes

A

Homologous chromosomes possess the same genes at the same loci (locations) because they are matched pairs—one inherited from each parent—but they do not necessarily have the same alleles. While they share the same structure and gene order, the specific alleles (versions of a gene) at a particular locus may be identical or differen

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20
Q

sister chromatids

A

sister chromatids of duplicaated chromosomes have same alleles for each gene
Prior to cell division, the DNA is replicated
 At the end of DNA replication, a duplicated
chromosome consists of two identical DNA double
helices, called sister chromatids, which are
attached to each other at the centromere

= Sister chromatids are two identical copies of the same chromosome that are joined together.

They form when DNA is copied

They are attached at the centromere

They carry the same genes

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21
Q

what happens to sister chromatids during mitotic cell divison?

A

During mitotic cell division, the two sister chromatids
separate, each becoming an independent
chromosome that is delivered to one of the two
daughter cells

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22
Q

Replication
and
Compaction of
Chromosomes
into Pairs of
Sister
Chromatids process

A

1) each chromosomes replicates before mitosis
2)at the start of mitsoisd, the chromosomes become compact

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23
Q

sequence of events from one cell division to the next

A

Process by which genetic material is duplicated and passed from parent cell to daughter cell;
- Each daughter cell receives a complete set of hereditary info. and half its cytoplasm from parent
cell Hereditary information usu. Identical to parent cell.

Process: The cell cycle (mitosis + cytokinesis)

“Genetic material is duplicated”
→ Interphase (S phase) — DNA is copied

“Passed from parent cell to daughter cell”
→ Mitosis — chromosomes are separated

“Each daughter cell receives a complete set of hereditary information”
→ Mitosis

“Half its cytoplasm from the parent cell”
→ Cytokinesis

“Hereditary information usually identical to parent cell”
→ Mitosis (produces identical cells)

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24
Q

stages of the cell cycle

A

Consists of two (2) main stages: Interphase and Mitotic Cell Division.
1. Growth Stage (Interphase) - acquisition of nutrients, growth, and chromosome duplication.
Cell grows in size, replicates DNA, and differentiates. Involves three (3) phases: G1, S and G2.
1. Growth 1 (G1) Phase: acquisition of nutrients and growth
2. Synthesis (S) Phase: DNA synthesis, every chromosome is replicated
3. Gap 2 or Growth 2 Phase (G2): completion of cell growth and preparation for division

  1. Cell Division (Mitotic Cell Division) – one parental cell divide into two daughter cells and Involves two (2) main
    processes: Mitosis and Cytokinesis
    Mitosis – Involves the division of the nucleus Content of nucleus divides and separate into two complete sets.
    Cytokinesis – Cytoplasm and organelles divide into two separate cells (daughter cells
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G1, S, G2
Growth 1 (G1) Phase: acquisition of nutrients and growth 2. Synthesis (S) Phase: DNA synthesis, every chromosome is replicated 3. Gap 2 or Growth 2 Phase (G2): completion of cell growth and preparation for division
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Cytokinesis vs mitosis
Mitosis – Involves the division of the nucleus Content of nucleus divides and separate into two complete sets. Cytokinesis – Cytoplasm and organelles divide into two separate cells (daughter cells)
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features of a eukaryotic chromosome before and after DNA replication
Before DNA replication (G1 phase) Each chromosome consists of one DNA double helix The chromosome is unduplicated It is a single chromatid Not yet paired with a sister chromatid 👉 In short: 1 chromosome = 1 DNA double helix After DNA replication (S phase, before mitosis/meiosis) Each chromosome is now duplicated It consists of two identical sister chromatids The sister chromatids are: Each made of one DNA double helix Held together at the centromere Still considered one chromosome until separation 👉 In short: 1 chromosome = 2 identical DNA double helices (sister chromatids) After sister chromatids separate (during anaphase) Sister chromatids separate Each chromatid becomes an independent daughter chromosome Each daughter chromosome has: One DNA double helix Identical genetic information 👉 In short: Separated sister chromatids = independent chromosomes
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Mitotic Cell Division
Mitotic cell division takes place in all eukaryotic organisms * It is the mechanism of asexual reproduction * Mitotic cell division followed by differentiation of the daughter cells allows a fertilized egg to grow into an adult with perhaps trillions of specialized cells * It allows organisms to maintain, repair, and even regenerate body parts
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stem cells
mitosis: It is the mechanism whereby stem cells reproduce.
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Mitosis has four main phases
Prophase Metaphase Anaphase Telophase
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prophase
Chromatin condensed into chromosomes; * Nuclear membrane breaks down and chromosomes releases into cytoplasm; * Nucleolus disappears; * Centriole move to opposite poles; * Spindle apparatus form between them “Centrioles move to opposite poles” Centrioles are small structures that help organize cell division During prophase, the two centrioles move away from each other They go to opposite ends (poles) of the cell 👉 This sets up the “left side” and “right side” of the cell so things divide evenly. “Spindle apparatus forms between them” As the centrioles move apart, spindle fibers (thin protein threads) form These fibers stretch from one centriole to the other The spindle fibers will later attach to chromosomes 👉 Their job is to pull chromosomes apart during division.
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Metaphase
Chromosomes is at the equator of the cell; * Centrioles are at each poles of the cell.
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Anaphase
Centromere split and chromatids split from each other; * Spindle fibers shorten and chromatids move to opposite poles; * At the end, one diploid set of chromosomes are at each end of the cell.
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Telophase
Begins when chromatids reach opposite poles of the cell; * Chromatids unwind into longer strands of chromatin; * Spindle fibers break down; * Nuclear membrane forms around new set of chromosomes * Nucleolus forms within each new nucleus.
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late interphase
Late Interphase Duplicated chromosomes are in the relaxed uncondensed state; duplicated centrioles remain clustered.
36
early prophase chromosomes
Early Prophase Chromosomes condense and shorten; spindle microtubules begin to form between separating centriole pairs
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late prophase
Late Prophase (also called Prometaphase) The nucleolus disappears; the nuclear envelope breaks down ALLOW some spindle microtubules attach to the kinetochore (blue) located at the centromere of each sister chromatid which will pull it apart later
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metaphase
Kinetochore microtubules line up the chromosomes at the cell’s equator.
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mitotic cell division in an animal cell (after metaphase)
Anaphase Sister chromatids separate and move to opposite poles of the cell; polar microtubules push the poles apart. Telophase One set of chromosomes reaches each pole and begins to decondense; nuclear envelopes start to form; nucleoli begin to reappear; spindle microtubules begin to disappear; microfilaments form rings around the equator. Cytokinesis The ring of microfilaments contracts, dividing the cell in two; each daughter cell receives one nucleus and about half of the cytoplasm. Interphase of daughter cells Spindles disappear, intact nuclear envelopes form, and the chromosomes extend completely.
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cytokinesis
Cytoplasm and organelle divide equally;  Indentation forms in cell membrane;  Indentation deepens until cell is pinched into two daughter cells;  Daughter cells are in G1 of Interphase. Chromosome number is maintained in mitosis because sister chromatids separate and are evenly distributed to opposite poles before cytokinesis, so each daughter cell receives a complete diploid set of chromosomes. Mitosis separates sister chromatids; meiosis I separates homologous chromosome pairs.
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Comparison between Cytokinesis in plant and animal cells are:
Comparison of Cytokinesis in Plant and Animal Cells Plant Cells Cytokinesis occurs by cell plate formation Vesicles from the Golgi apparatus gather at the center These vesicles fuse to form a cell plate The cell plate grows outward and becomes the new cell wall No cleavage furrow forms Animal Cells Cytokinesis occurs by cleavage A cleavage furrow forms at the cell’s equator The plasma membrane pinches inward Contractile ring of actin and myosin helps separate the cells No cell wall is formed
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Importance and Regulation of the Cell Cycle
Important for growth, repair and maintenance of tissues; However, division must be regulated: * Regulated by regulatory signals that ‘start’ and ‘stop’ signals; * Rapid uncontrolled cell division causes Cancer
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Meiosis two main outcomes
Reduction Division - produces daughter cells with half the no. of chromosomes as parent cells; 2. Recombination – daughter cells produced has diff. combination of genes, hence differ from each other and parent Purpose of Meiosis I – “HALF & MIX” Applied idea: Meiosis I is all about reducing chromosome number and creating genetic diversity. Reduces chromosome number: Homologous chromosomes (mom + dad) separate Results in haploid cells (n), so gametes won’t double chromosome number at fertilization Mixes genetic material: Crossing over swaps DNA between homologous chromosomes Random alignment during metaphase I → independent assortment Creates unique combinations for offspring Example in real life: In humans, meiosis I ensures that sperm or egg cells each have 23 chromosomes, and each gamete is genetically unique. Purpose of Meiosis II – “SPLIT & FINALIZE” Applied idea: Meiosis II is all about separating sister chromatids to make functional gametes. Separates sister chromatids: Each haploid cell from meiosis I divides again Produces four haploid gametes in total Finalizes genetic content: Each gamete has one copy of each chromosome Ensures correct chromosome number when fertilization occurs Example in real life: In females: meiosis II produces one functional ovum In males: meiosis II produces four functional sperm cells
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meisosis
During sexual reproduction, gamete from male fuses with gamete from female to form zygote; * Zygote has genetic info. from both parents (half from mother and half from father); * Process that produces haploid gametes is Meiosis
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meiotic cell division process
2n--n--2n 2n--n--2n both meet at the 2nn, 2n first = diploid parental cells, n=haploid gametes, become 2n via fertilization
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4 phases of meiosis I
Prophase, Metaphase, Anaphase and Telophase  Involves two (2) complete cycles of the above phases
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meiosis I and II
MeiosIs I - separates homologous chromosomes, to produce haploid daughter nuclei. Meiosis II - Meiosis II separates sister chromatids into four daughter nuclei
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Prophase I, Meiosis I
Prophase 1 - Homologous chromosomes pair up and exchange DNA (synapsis); - Crossing Over occurs; - During synapsis: * Homologous chromosomes pair up; * Each chromosomes consists of two chromatids * Hence, pair of homologous chromosomes consists of four chromatids (tetrad).
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crossing over/prophase I
Exchange of sections of chromatids between maternal and paternal homologues; — Enzymes cut through DNA of paired homologues and graft cut ends back together, switching maternal and paternal ends; — Leave crosses or chiasmata (singular, chiasma), where maternal and paternal chromosomes have exchanged parts;  Result in chromosomes containing genes of maternal and paternal origin.
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metaphase I, meiosis I
Metaphase 1 - Paired homologous chromosomes line up at the equator of the cell; - Spindle fiber attaches to centromere of each homologous chromosomes; - Guide each tetrad to the equator of the cell
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independent assortment
Duplicated homologous chromosomes are pulled perpendicular to the spindle. A daughter cell receive any combination of maternal and paternal homologues  This random combining of maternal and paternal homologues (and, therefore, alleles) is called independent assortment
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genetic diversity/haploid cells
Genetic recombination and independent assortment are responsible for genetic diversity of the haploid cells produced by meiosis.
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Anaphase I, Meiosis I
Anaphase 1 - Spindle fibers shorten: - Whole duplicated chromosomes of each homologous pair separate from each other; - One duplicated chromosome of each homologous pair moves to each pole, pulled by microtubules - Sister chromatids still held by centromere; - Single chromosome (2 sister chromatids) from each homologous pair move to opposite pole of ce
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Telophase 1
Homologous chromosomes reach opposite poles ↓ Two haploid clusters of duplicated chromosomes form ↓ Homologous chromosomes begin to uncoil ↓ Spindle fibers disappear ↓ Nuclear membrane forms around each chromosome cluster ↓ Cytoplasm divides (cytokinesis) ↓ Two haploid cells are formed (each cell has one set of duplicated chromosomes)
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Meiosis II
Telophase I is usually followed immediately by meiosis II, with little or no intervening interphase
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Independent Assortment
Occurs during Metaphase I;  Chromosomes arranged in homologous pairs along equator;  Maternal chromosomes faces one pole of the cell and paternal chromosomes faces the other pole;  Result in gametes with diff. combinations of maternal and paternal chromosomes
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crossing over
Occurs during Prophase I;  Homologous chromosomes pair upside by side;  Non-sister chromatids may exchange pieces of chromosomes (crossing over);  Result in chromosomes containing genes of maternal and paternal origin
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Meiosis II phases
Cell goes through prophase II, metaphase II, anaphase II, and telophase II as in Mitosis;
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primary function of meosis II
Meiosis II separates sister chromatids into four daughter nuclei; o Daughter cells are haploid and contain single replicated chromosome.
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Prophase II, Meiosis II
Prophase II o If decondensed, chromosomes recondense o Spindle microtubules re-form and capture duplicated chromosomes to pull them to opposite sides of the cell
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Metaphase II
Metaphase II o Duplicated chromosomes line up singly, perpendicular to the spindle
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Anaphase II
Anaphase II o Chromatids separate and move to opposite poles
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meiosis I
1️⃣ Prophase I (the long one) This is the big event stage. What happens: Chromosomes condense (you can see them) Homologous chromosomes pair up (one from mom, one from dad) → called synapsis They form tetrads (4 chromatids total) Crossing over happens → chromatids swap pieces = genetic diversity Nuclear membrane breaks down Spindle fibers form 🧠 Key idea: DNA is mixed and matched here 2️⃣ Metaphase I What happens: Paired homologous chromosomes line up at the middle of the cell Alignment is random (independent assortment) 🧠 Key idea: which parent’s chromosome goes where is random 3️⃣ Anaphase I What happens: Homologous chromosomes separate One whole chromosome (still 2 sister chromatids) goes to each side Sister chromatids stay together 🧠 Key idea: chromosome number is reduced 4️⃣ Telophase I + Cytokinesis What happens: Chromosomes reach opposite poles Cell divides into two cells Each cell is haploid (n) Chromosomes are still duplicated (sister chromatids attached) 🧠 Key idea: two genetically different haploid cells are made ⭐ One-line memory trick Meiosis I separates homologous chromosomes (Meiosis II separates sister chromatids)
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Telophase II
Cytokinesis occurs, nuclear membranes re-form, and chromosomes decondense o The two nuclear divisions of meiosis produce four haploid cells from a single diploid cell.
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result of cytokinesis meisosis II
Cytokinesis results in four haploid cells, each containing one member of each pair of homologous chromosomes (shown here in the condensed state).
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comparison of Meiosis I with mitosis across phases
meisosi I, prophase I: pairing of homologous chromosomes mitosis, prophase: no pairing of chromosomes metaphase I, meiosis: homologous duplicated chromosomes at metaphase plate Metaphase, Mitosis: duplicated chromosomes at metaphase plate Anaphase I, Meiosis: homologous chromosomes separate Anaphase: sister chromatids separate, becoming daughter chromosomes that move to the poles Telophase I, Meiosis: two haploid daughter cells telophase, cytokinesis, mitosis: two daughter cells, identical to the parental cell
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haploid vs diploid
haploid=n diploid=2n adults=2n meotic cell division in ovaries/testes: egg (n) and sperm (n/0 fusion of gametes= zygote (2n) embryo= 2n baby=2n
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Nondisjunction
Process in which chromosomes or sister chromatids do not separate. Occurs in Anaphase I and Anaphase II Anaphase I - homologous chromosome pairs do not separate to opposite poles, BUT one pair pulled towards same pole; Anaphase II - sister chromatids do not separate to opposite poles BUT both sister chromatids pulled toward same pole.
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consequences of nondisjunction
Produces gametes with too few or too many chromosomes. Effects of Nondisjunction 1. Monosomy - one chromosome is lost. E. g. Turner Syndrome (X chromosome is missing). 2. Trisomy – one chromosome is gained. E. g. Down syndrome (extra chromosome # 21).
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turner syndrome, poly-x and xxy, jacobs syndrome
Normal XX females have one X chromosome inactive. * A zygote with one X chromosome (Turner syndrome) can survive. – All extra X chromosomes become deactivated. * Poly-X females and XXY males are seen fairly frequently. – Extra Y chromosomes are also tolerated. * Jacobs syndrome (XYY) is due to nondisjunction during meiosis II of spermatogenesis.
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Patau and Edwards syndrome
can be either male or female patau: trisomy 13, chromosome 47 edwards: trisomy 18. chromosome 47
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klinefelter/Turner Syndrome
klinefelter: male, XXY or XXXY, chromosome 47 or 48 turner: female only, chromolsomes X, chromosome 45
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jacobs syndrome
malw only, chromosomes=XYY, chromosome 47
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symptoms of down, edward, patau
down: mental retardation, abnormal pattern of palm creases, slanted eyes, flattened face, short, early death edward: mental and physical retardation, facial abnormalitiies, extreme muscle tone, early death patau: mental and physical retardation, wide variety of defects in organs, large triangular nose, early death
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turner syndrome symptoms
short stature, webbed neck, sexually undeveloped
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klinefelter symptoms
sexual immaturity, breast swelling in males XXY
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down syndrome
Down Syndrome – trisomy 21 — Most common autosomal trisomy among humans — Easily recognized physical features — Chances of having a Down syndrome child increase rapidly with age, starting at about age 40 — Some symptoms may be due to expression of Gart gene  Mild to severe mental impairmen
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changes in sex chromosome number
An abnormal sex chromosome number is caused by inheriting too many or too few X or Y chromosomes.  Nondisjunction during oogenesis or spermatogenesis results in gametes with too few or too many X and Y chromosomes.  Fertilization of the gametes can lead to various syndromes.
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prescence of Y chromosomes
Presence of Y chromosome, not the number of X, determines maleness. — SRY gene produces a hormone, testis- determining factor — Plays a key role in male genital development
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Turner syndrome
Females have one X chromosome.  Usually are short with malformed features, such as webbed neck, high palate, and small jaw  Many have congenital heart and kidney defects  Most have ovarian failure and do not undergo puberty or menstruate without hormone therapy.
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klinefelter/diagnosis
A male with 2 X and one Y chromosome  Subtle symptoms; usually not diagnosed until age 15  Usually experience speech and language delays  Require assisted reproduction to father children
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Poly X Females or Triplo-X
Poly-X females have more than two X chromosomes. — They are usually taller, with no other distinctive phenotypes. — Some have delayed motor and language development. — Some may have menstrual difficulties, but most do so regularly and are fertile. — Their children usually have normal karyotypes.
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Jacobs Syndrome
XYY males result from nondisjunction during spermatogenesis. — Males are usually taller, suffer from persistent acne, and tend to have speech and reading abnormalities. — There is typically no behavioral differences between an XY and XYY male.
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spermatogenesis/oogenesis
Gametes are Sperm and Egg. Spermatogenesis - Production of sperm Oogenesis – Production of egg. Both processes involves Meiosis to produce haploid gametes.
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spermatagonia
Spermatogonia divide by mitosis to form 2 daughter cells:  One daughter cell replenish spermatogonia population;  Next daughter cell develops into Primary Spermtocyte.  Primary spermatocyte undergo meiosis I to form two Secondary spermatocytes:  Secondary spermatocytes undergo meiosis II to form four spermatids;  Spermatids mature into sperm.
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process of sperm production
mitossis, meiosis I, meiosis II, differentiation GERM CELLS CAN DO MITOSIS, SEX CELLS DONT
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Oogenesis
Starts with a diploid cell (Oogonium);  Each oogonium goes through mitosis to form 2 primary oocytes:  1° oocyte remain until puberty.  Every month after puberty, one 1° oocyte undergo meiosis I:  Involves unequal division of cytoplasm (asymmetrical cytokinesis)  Form two daughter cells: Secondary oocyte (larger cytoplasm) and first polar body.  First polar body non-functional and disintegrate.
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secondary oocyte
Secondary oocyte undergo meiosis II:  Cytoplasm is unequally divided to form two daughter cells;  Cell with most cytoplasm, contain nutrients and becomes an egg/ovum;  Other cell, second polar body disintegrate.
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process of oogensesis
Oogonium (2n) │ Mitosis ▼ Primary oocyte (2n) │ Meiosis I (asymmetric division) ▼ Secondary oocyte (n) + First polar body (n) │ Meiosis II (only if fertilization occurs) ▼ Ovum / Egg (n) + Second polar body (n) 🧠 Key things to remember (exam gold): Only ONE functional egg is produced Polar bodies get rid of extra chromosomes Oogenesis results in 1 ovum + 3 polar bodies Meiosis II only finishes if fertilization happens
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primary oocytes
Primary oocytes begin Meiosis I before birth; - BUT remains in Prophase I until puberty; - At puberty, hormone signal 1°oocyte to resume Meiosis I; - Meiosis I is completed; - 2° oocyte released and travel down Fallopian tube and remains in Metaphase II until fertilization
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if fertilization does not occur/oocytes
If fertilization does not occur, 2° oocyte will NOT complete Meiosis II; - If fertilization occurs, 2° oocyte complete Meiotic II: - Produces an ovum or egg cell and a polar body; - Haploid egg fuses with haploid sperm to form a zygote
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sexual reproduction
Organs called gonads produce haploid sperm or eggs through meiotic cell division;  Female gonad, called the ovary, produces eggs;  Male gonad, called testis (plural, testes), produces, motile sperm.  Sperm and egg fuse (fertilization) to form a zygote >>>>mitotic cell divisions to produce offspring;  Offspring is not genetically identical;  Genetic variability allow offspring to survive (exploit new habitats, acquire food and avoid predation)
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asexual reproduction
Asexual Reproduction  Single animal produces offspring, through repeated mitotic cell division in some part of its body;  offspring are genetically identical to the parent;  Org. reproduces without mating;  Efficient in effort: no need to search for mates, court members of opposite sex, or battle rivals, no wasted gametes and offspring have all the genes of their parent;  Several common methods of asexual reproduction in the animal kingdom are: Budding, Fission and Parthenogenesis.
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major forms of reproduction
Binary Fission; 2. Conjugation; 3. Budding; 4. Vegetative Reproduction; 5. Fragmentation; 6. Parthenogenesis and 7. Spore Formation
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binary fission/conjugation
Binary Fission - A method of asexual reproduction that involves the splitting of a parent cell into two daughter cells. 2. Conjugation – transfer of genetic material from one cell to another through a bridge called a Pilus.
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budding
Asexual Reproduction - Budding Miniature version of parents grow out from parent’s body to form a complete new individual; Cells from parent undergo mitosis; Differentiate into specific types of structures before the new individual breaks away from the parent; Continues throughout an animal’s lifetime.
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fragmentation
Fragmentation  Creation of new plant from a fragment/portion of parent plant, E. g Potatoes
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Asexual Reproduction – Parthenogenesis
Eggs develop into viable offspring without fertilization;  In some species, offspring are haploid; E. g. Honeybees, queen bee lays both fertilized and unfertilized eggs:  Fertilized eggs develop into male worker bees;  Unfertilized eggs develop into male drones.
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spore formation
Spores – structure that contain genetic material and cytoplasm surrounded by a wall: * Small and dispersed by wind or water; * Walls protect spore content until favorable cond'tn; * Wall opens and org. begin to develop.  Spores may be haploid or diploid;  Some spore may be produced by meiosis:  Result in alternation of generations
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Life cycles of plants which consists of two generations: Diploid and Haploid generation.
Life cycle involves a Sporophyte (diploid) generation and a Gametophyte (haploid) generation: * Sporophyte generation produces haploid spores by meiosis; * Spores develop and grows into a plant body, Gametophyte; * Gametophyte produces male and female gametes; * Gametes fuse at fertilization to form another sporophyte; * Cycle continues. * Found only in plants, except Phylum Cnidaria (jelly fish, sea anemone
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gametophyte/stalks
Gametophyte is Leafy green mat;  Stalks (Sporophyte) grows on mat and releases spores from its cap;  Spores fall to ground and develop into leafy Gametophyte;  Gametophyte develop structures that produces sperm and eggs;  Sperm swim to the eggs and fertilize them;  Fertilized eggs developed into new stalk  Cycle continues
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conifers
Sporophyte stage (2n) – the tree itself The conifer tree is the diploid sporophyte. It produces two types of cones: Male cones (smaller) → produce pollen Female cones (larger) → produce ovules Gametophyte formation (n) Female cones: Inside each scale, meiosis occurs → haploid spores are produced. These spores develop into female gametophytes, but stay inside the ovule (protected). Male cones: Inside each scale, meiosis produces haploid spores. These develop into male gametophytes (pollen grains). Pollen is released into the air for dispersal. Fertilization Pollen grains reach the female gametophyte. Sperm (from pollen) fertilizes the egg (in female gametophyte) → forms zygote (2n). Seed formation The zygote grows into an embryo inside the seed. Seed can eventually grow into a new sporophyte tree.
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advantages vs disadvantages of reproductive strategies
Advantages of Sexual Reproduction Advantages of Asexual Reproduction Offspring adapt to changing environment Proceeds quickly and do not need a second parent Reduced competition among siblings as they are genetically diverse Requires less energy Damaged chromosomes can be replaced or repaired Greater no. of offspring
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difference in outcomes between meisosis I and II
Meiosis I – “HALF & MIX” Outcome: Chromosome number is cut in half Produces 2 haploid cells Chromosomes are still duplicated Cells are genetically different 🧠 Acronym: HALF Halve chromosome number Altered genes (crossing over) Leaves sister chromatids together Forms 2 cells Meiosis II – “SPLIT” Outcome: Chromosome number stays the same Produces 4 haploid cells total Sister chromatids separate Cells are genetically different 🧠 Acronym: SPLIT Sister chromatids separate Produces 4 total cells Leaves chromosome number the same Identical? ❌ (still different) Two divisions total (including meiosis I)
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difference bewteen secondary oocyte and mature ovum
Each cycle, one secondary oocyte (n) is released from the ovary during ovulation. This cell has completed meiosis I. It is arrested at metaphase II of meiosis II. This is what is usually called the “egg” that is ovulated — technically, it’s not yet a mature ovum.
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how does mitosis form 2 diploid cells?
by replicating DNA in the S interphase first, then seperating sister chromatids during anaphase
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how does meiosis I form two haploid cells?
by spewrating homologous chromsomes = reduces the chromosome count, , nuclear membranes reform to produce two haploid cells
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how does meiosis II form 4 haploid cells?
Meiosis II separates sister chromatids of the two haploid cells from meiosis I, producing four haploid daughter cells, each with one copy of every chromosome.
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when do chromosomes vs chromatids exist
chromomsomes= throughouyt the cell cycle chromatids: exist only afeter the s phase of reeplication
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golgi apparatus, endoplasmic reticulum, lysosome, centriole functions
Golgi apparatus: Modifies, sorts, and packages proteins and lipids for transport to their destinations in or out of the cell. Endoplasmic reticulum: Synthesizes proteins (rough ER) and lipids (smooth ER) and helps transport them within the cell. Lysosome: Breaks down waste materials, damaged organelles, and macromolecules using digestive enzymes. Centriole: Organizes microtubules and helps form the spindle fibers during cell division.
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Three Domains of Eukaryotic Org.
plants, fungi, animals
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3 parts of The three main parts of the eukaryotic nucleus are:
Nuclear envelope Nucleolus Chromatin
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levels of organization of genetic material
1. DNA double helix 2. DNA winds around histine proteins 3. has coiled DNA and hisdtoine beads 4. loops attach to ptrotein scaffold: this typically occurs in cells that are not dividing 5. results in a fully folded chromosome
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genetic recombination
crossing over independent assortment
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vegetative reproduction
Vegetative reproduction, or propagation, is a form of asexual plant reproduction where new, genetically identical plants (clones) grow from a parent plant's vegetative parts like roots, stems, or leaves, rather than seeds or spores, using natural methods (rhizomes, tubers, bulbs) or artificial techniques (cutting, grafting, layering) for rapid, uniform multiplication
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Alternation of Generations
Life cycle involves a Sporophyte (diploid) generation and a Gametophyte (haploid) generation: * Sporophyte generation produces haploid spores by meiosis; * Spores develop and grows into a plant body, Gametophyte; * Gametophyte produces male and female gametes; * Gametes fuse at fertilization to form another sporophyte; * Cycle continues. * Found only in plants, except Phylum Cnidaria (jelly fish, sea anemone
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alternation of generations in moss
Gametophyte is Leafy green mat;  Stalks (Sporophyte) grows on mat and releases spores from its cap;  Spores fall to ground and develop into leafy Gametophyte;  Gametophyte develop structures that produces sperm and eggs;  Sperm swim to the eggs and fertilize them;  Fertilized eggs developed into new stalk
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humans have x autosomes
22 autrosomes, 1 sex chromosome pair
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difference between kinetochore microtubules/spindles
Location: Spindle fibers extend between spindle poles (centrosomes) and chromosomes. Kinetochores are located on the centromere of each sister chromatid. Function: Spindle fibers act as "ropes" that guide and move chromosomes. Kinetochores act as the attachment site for these ropes, facilitating binding and tension monitoring.
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zygote that exists but doesnt implant =produces hcg, but then fails to implant
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differentiation
respiratory system
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1st, 2nd, 3rd cell division
1st division= 2 cells 2nd dvision=4 cells 3rd division= 8 cells only number of cells incrfeases, not mass MORULA=16 CELLS
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inner mass cells vs trophoblasts
trophoblasts =become chorion inner mass cells= embryo
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In conifers, the 'pollen grain' represents which stage of the life cycle? Which of these is the correct sequence for the development of a moss plant? Which transition represents the switch from the gametophyte generation to the sporophyte generation?
male gametophyte, which prodcuces male gamete spore, gametophyte, GAMETES, ZYGOTE, sphorophyte fertilization
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mitosis
type of cell division gone wrong in cancer
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cytoskeleton
transport from one place to another, structure of cell
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nuclear envelope
double membrane nucelaus houses DNA, carries blueprints for making proteins
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endomembrane system
protein processing occurs here; cells network of internal membranes rough ER: covered in ribosomes, makes it rough no endomembrane system in prokaryotes
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extremophiles
live in almost any temp/bacteria
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first cell on earth
went onto to produce protocell, which then produced cyanobacteria, archea, then eukarya
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euykaryotic
plants, animals and protists
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23n
= 46 total chromosomes
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adenine complementary to
thymine
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antiparallel direction
how each strand runs with one another
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mother cell
first cell, daughter cells are offspring
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mitosis
occurs in all living organisms= naisl grow back bc of mitosis
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plant cell
has cytokinesis, but doesnt pinch, forms the cell plate NO CENTRIOLES IN PLANTS BUT STILL HAS SPINDLE FIBERS
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ONCO GENES
control cell divison
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During G1, a daughter cell can:
✅ Increase in size (grow and make proteins/organelles) ✅ Differentiate (specialize in structure and function, depending on the cell type) ✅ Respond to internal and external signals to decide whether to: Move forward to the S phase (DNA replication), or Exit the cycle into G₀ (a resting/non-dividing state)
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Beginning with a newly formed daughter cell, the order of the stages of the eukaryotic cell cycle is:
G1 → S → G2 → M
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G1 'checkpoint'
So the G1 → S checkpoint makes sure: The cell is large enough There are enough nutrients/resources The DNA is undamaged Conditions are favorable for division
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The G2 → M checkpoint ensures
DNA was copied correctly (no damage or mutations) The cell is large enough and has enough organelles and proteins for division The cell is ready to enter mitosis safely
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The metaphase → anaphase checkpoint
all chromosomes are properly aligned at the metaphase plate Spindle fibers (microtubules) are correctly attached to the kinetochores of each chromosome Sister chromatids are ready to separate without errors
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steps in cytokinesis
1. Actin filaments form a ring around the cell’s equator. 2. The contractile ring contracts, pinching in the cell’s “cleavage furrow.” 3. he “cleavage furrow” completely pinches off, forming two daughter cells
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steps in plant cytokinesis
Carbohydrate-filled vesicles bud off the Golgi apparatus and move to the center of the cell. The vesicles fuse to form a new cell plate and plasma membrane between the two daughter cells. Complete separation of the two daughter cells.
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when does meiosis start
Key point: Meiosis is part of the cell cycle for germ cells, and it starts after DNA replication in S phase,
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telomeres
The terminal ends or "tips" of a chromosome are called telomeres.
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role of resulting cells from meisosi and mitosis
mitosis: body functions meiosis: sesxual functions
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visual appearance of interphase, early proiphase vs late prophase
interphase: still has nucleaulos early prophase: no nucleaulus, chromatin starting to condense late propahse: looks like a hybrid between anaphase/metapahase
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way to tell mitosis prophase from meiosis prophase
mitosis prophase early: no condensed chromosomes meiosis prophase: homologous chromosomes already formed meiosis 2 prophase: sister chromatids aslready fromed