What is the difference between magnification and resolution?
Magnification is the how much bigger the image you see is than the real object.
Resolution is the measure of how close two objects can be before they are seen as one.
What are the five main components of the light microscope?
Eyepiece lens
Objective lens
Specimen
Stage
Light source
What are the advantages of the light microscope?
-can see live pathogen so can compare with living tissue
-relatively cheap so available to many people
-relatively light and portable so can be used almost anywhere
What are the disadvantages of a light microscope?
-limited resolution and magnification
-require a stain so can cause the observation to be a result of preparation rather than a true representation of the living tissue
What is a transmission electron micrograph?
Micrographs produced by the electron microscope that gives 2D images like those of a light microscope but magnified up to 500000 times
What is a scanning electron micrograph?
Are micrographs produced by the electron microscope that have a lower magnification than TEMs but produce a 3D images
What is the magnification equation?
Image size = actual size x magnification
What is a light microscope?
A tool that uses a beam of light and optical lenses to magnify specimens up to 1500 life size
What is an electron microscope?
A tool that uses a beam of electrons and magnetic lenses to magnify specimens up to 500000 times life size
What are the advantages of electron microscopes?
Huge powers of magnification and resolution so can see many cell structures
What are the disadvantages of electron microscopes?
-extremely expensive
-only magnify dead pathogens
-all specimens examined in a vacuum
-large and kept at a constant temperature
