Which organelles don’t have membranes?
• Ribosomes
• Cytoskeleton
• Centrioles
How big are ribosomes?
About 20nm
How are ribosomes structured?
• Small
• Spherical
• Made of ribosomal RNA
Where and how are ribosomes made?
In nucleolus as two separate subunits, passing through nuclear envelope into cell cytoplasm and then combine
Where do ribosomes end up in a cell?
• Some remain free in cytoplasm
• Some attach to endoplasmic reticulum
What function do ribosomes bound to the RER exterior have?
Mainly synthesise proteins that will be exported outside cell
What function do ribosomes have in the cytoplasm?
Singly or in clusters, primarily site of assembling proteins used in cell
How are centrioles structured?
• Consist of two microtubule bundles at right angles
• Made of tubulin protein subunits, arranged to form a cylinder
What is the function of centrioles in mitosis?
• Before dividing, spindle forms from centrioles
• Chromosomes attach to spindle middle and motor proteins walk along tubulin threads, pulling chromosomes to opposite cell ends
What is the spindle in a centriole made from?
Threads of tubulin
How are centrioles involved in forming cilia and undulipodia?
• Before cilia form, centropes multiply and line up beneath cell surface membrane
• Microtubules then sprout outwards from each centriole forming a cilium/undulipodium
Where are centrioles usually absent from?
• Cells of higher plants
• May be in some unicellular green algae
What is a cytoskeleton?
A network of protein structures within cytoplasm
What does the cytoskeleton contain?
• Rod-like microfilaments made of protein actin subunits
• Intermediate filaments
• Straight, cylindrical microtubules
• Cytoskeleton motor proteins
What is a microfilament?
Polymer of actin
How big is each microfilament?
7nm in diameter
How big are intermediate filaments?
10nm in diameter
What are straight cylindrical microtubules made from?
Protein subunits called tubulin
How big are straight cylindrical microtubules?
18-30nm
What are 3 molecular motors?
• Myosins
• Kinesins
• Dyneins
What are molecular motors?
• Enzymes
• Have site binding and allowing hydrolysis of ATP as energy source
What do the protein microfilamnets in cytoplasm do?
• Give support
• Give mechanical strength
• Keep cell’s shape stable
• Allows cell movement
What do microtubules provide for a cell?
• Shape and support
• Help substances and organelles to mive through cytoplasm
How do microtubules allow organelles to move in the cytoplasm?
• Form track along which motor proteins walk and drag organelles from one cell part to another
• Form spindle before cell divides, enabling chromosomes to be moved