p36(22)

Question 1

culture media According to Composition

Answer 1

• Synthetic or Defined Medium
• Non - Synthetic Medium
• Tissue culture Medium

Question 2

culture media According to Composition
• All components are known
• For research purposes cyanobacteria (e.g. and chemoorganotrophs)

Answer 2

Synthetic or Defined Medium

Question 3

Synthetic or Defined Medium example

Answer 3

BG -11 medium

Question 4

culture media According to Composition
• Some components are unknown (peptones, meat and yeast extract)
• For the isolation of medically important bacteria

Answer 4

Non - Synthetic Medium

Question 5

Non - Synthetic Medium example

Answer 5

•nutrient broth medium, NB
•trypticase soy broth TSB,
•MAC agar

Question 6

culture media According to Composition
• Used for obligate intracellular bacteria
(Rickettsia and Chlamydia)

Answer 6

Tissue culture Medium

Question 7

Tissue culture Medium example

Answer 7

•W138 cells,
•HeLa 229 cells and
•McCoy cells (For chlamydia)

Question 8

human cervical tissue cells

Answer 8

HeLa cells

Question 9

W138 cells and McCoy cells are

Answer 9

fibroblasts

Question 10

culture media According to the Dispensing or Distribution

Answer 10

• Plated
• Tube

Question 11

CM (poured in a dish or plates)

Answer 11

Plated

Question 12

CM (liquid, slant, butt and slat or butt)

Answer 12

Tube

Question 13

Tube culture media ex

Answer 13

triple sugar iron TSI,
sulfide indole motility SIM and
lysine iron agar LIA

Question 14

CM According to Usage

Answer 14

•Simple Media | General Purpose Media and Supportive Media
• Enrichment Media
• Enriched Media
• Differential Media

Question 15

•ROUTINELY used as it can support most non - fastidious bacteria
• Contains meat and soybean extracts

Answer 15

Simple Media | General Purpose Media and Supportive Media

Question 16

Simple Media | General Purpose Media and Supportive Media examples

Answer 16

Nutrient agar,
Nutrient broth, and
TSB

Question 17

•Promote growth of a certain growth of bacteria from a mixture of organism
•Contain specific nutrients WITHOUT additional supplements; following growth, colonies are sub-cultured to isolate desired organism

Answer 17

Enrichment Media

Question 18

Enrichment Media examples

Answer 18

Alkaline Peptone water,
Selenite

Question 19

CONTAIN ADDITIONAL SUPPLEMENT to support growth of fastidious organisms (Blood, vitamins and etc)

Answer 19

Enriched Media

Question 20

Enriched Media examples

BOTH are used to isolate fastidious organism

Answer 20

Blood Agar Plate (BAP) and
Chocolate Agar Plate (CAP)

Question 21

•used to classify hemolytic patterns of bacteria
•for hemolytic pattern identification

Answer 21

Blood Agar Plate (BAP)

Question 22

used for the recovery of Haemophilus spp.

Answer 22

Chocolate Agar Plate (CAP)

Question 23

Provides visualization of metabolic differences between
groups of bacteria

Answer 23

Differential Media

Question 24

Differential Media examples

Answer 24

MAC and
BAP,
EMB and
Hektoen Enteric Agar

Question 25

serves as pH indicator which detects fermentation of sugar and acid production

Answer 25

Neutral red in MAC

Question 26

Pink colonies: MAC

Answer 26

Lactose fermenter

Question 27

Colorless colonies: MAC

Answer 27

Non Lactose fermenter

Question 28

Contain antibiotics, dyes or chemicals which purposively used to prevent the growth of non-significant organisms leaving the significant ones healthy

Answer 28

Selective Media

Question 29

Selective Media examples

Answer 29

HEA, MAC, Xylose Lysine Desoxycholate (XLD), Bismuth Sulfate agar, Mannitol Salt Agar (MSA)

Question 30

For Streptococcus

Answer 30

Gentamicin containing:

Question 31

for Haemophilus

Answer 31

Bacitracin Chocolate

Question 32

for Aeromonas

Answer 32

Blood Agar Plate BAP with Ampicillin

Question 33

for gram positive bacteria

Answer 33

•Phenylethel alcohol •Columbia Colistin - Nalidixic acid CCNA