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Question 1

Very difficult to grow; requires special or additional requirements to grow in culture media

Answer 1

Fastidious organisms:

Question 2

Culture Media:

Answer 2

Blood Agar Plate

Question 3

Ist priority:

Answer 3

5% defibrinated sheep’s blood

Question 4

2nd priority:

Answer 4

horse blood

Question 5

3rd priority:

Answer 5

rabbit’s blood

Question 6

least priority:

Answer 6

human blood (must be type O - no presence of antigens)

Question 7

• Hernin/Hematin
•HEAT STABLE FACTOR released
after degrodation of hemoglobin during hemolysis

Answer 7

X factor

Question 8

• HEAT LABILE FACTOR
• Nicolinamide adenine dinucleotide (NAD)
• Coenzyme 1
• V factor is attributed to “vitamin”

Answer 8

V Factor

Question 9

Environmental factors influencing growth

Answer 9

•pH
• Temperature
• Gaseous composition of the atmosphere

Question 10

Most PATHOGENIC bacteria grow best at a neutral ph with a final pH adjusted between

Answer 10

7.0 and 7.5

Question 11

related cultures are incubaled at
30° C.

Answer 11

Mycoses

Question 12

can grow at 35 lo 42 °C

Answer 12

Pseudomonas aeruginosa
Campylobacter

Question 13

pH 0 to 5.5: Acid loving bacteria

Answer 13

Acidophiles
Example: Lactobacillus acidophius -
Normal flora of vagina

Question 14

•pH 5.5 to 8.0; Diagnostic laboratory media for bacteria are usually adjusted to a final pH between 7.0 and 7.5
•MOST CLINICALLY SIGNIFICANT
BACTERIA (6.5 TO 7,5)

Answer 14

Neutrophiles

Question 15

pH 8.5 to 11.5: Alkali loving bacteria
Example: Gordnerella vaginal

Answer 15

Alkalophiles

Question 16

Bacteria that grow best at cold temperatures: OT of 10 to 20°C

Answer 16

Psychrophiles
Cryophiles

Question 17

Bacieria that grow best at moderate temperatures; OT of 20 to 40°C;

Answer 17

Mesophiles

Question 18

Bacteria that grow best at high temperatures; OT of 50 to 60°C; to 125°C

Answer 18

Thermophiles

Question 19

Can survive at extreme environment like below earth’s surface (Bacillus infernus)

Answer 19

Extremophiles

Question 20

require and grow in increased sodium chloride
concentration

Answer 20

halophiles

Question 21

halophiles bacteria

Answer 21

Staphylococcus aureus and Listeria monocytogenes

Question 22

organism that grow rapidly in high pressure environments
600 to atmospheric pressure

Answer 22

Barophiles:

Question 23

Barophiles bacteria

Answer 23

Photobacterium Shewanella and Colwellia

Question 24

High Osmotic Pressure org

Answer 24

Osmophilic bacteria (Archaebacteria spp.)

Question 25

wide thermal range for growth

Answer 25

Eurithermophilic;

Question 26

narrow thermal range for growth

Answer 26

Sternothermophilic: