page 18

Question 1

Other Stains for Acid-fast Organisms

Answer 1

• Pappenheim stain
• Baumgarten stain

Question 2

Pappenheim stain
• Mycobacterium tuberculosis - ____
• Mycobactenum laclicolo (smegmatis) - _____

Answer 2

RED
BLUE

Question 3

uses rosolic acid as a decolorizer

Answer 3

Baumgarten stain

Question 4

Baumgarten stain - uses rosolic acid as a decolorizer
• Mycobacterium tuberculosis - ______
• Mycobacterium leproe - ___

Answer 4

BLUE
RED

Question 5

Leifson stain
Target Structure:
Color Reaction:

Answer 5

Flagella
Red/Purple

Question 6

Loeffer stain
Target Struclure:
Color Reaction:

Answer 6

Metachromatic granules
Dark blue or purple

Question 7

Loeffler’s Alkaline Methylene Blue (LAMB) stain
Target Struclure:
Color Reaction:

Answer 7

Metachromatic granules
Dark blue or violet

Question 8

Fulton-Schaeffer stain
Target Struclure:
Color Reaction:

Answer 8

Spores/Endospores
Spores are green

Question 9

uses special stains for differentiation of a specific part of the cell or specific microorganisms

Answer 9

Selective staining :

Question 11

demonstrates he presence of diffuse capsule surrounding some pacteria where bacterial cell appears light - colored against a dark background

Answer 11

Indirect/Negative/Rellef staining: