Scientific methodologies:
Sample location: random sampling?
Importance of the avoidance of bias.
Random sampling: if the study area has a regular shape then a grid can be laid out and the coordinates of sampling sites selected using random numbers. If the study area has an irregular shape then all the possible sampling sites can be numbered and selected using a random number generator.
Scientific methodologies:
Sample location: systematic sampling?
Regular sample intervals.
Transects – applied to ‘environmental gradients’:
line transects
belt transects
continuous/interrupted transects.
systematic sampling: this involves samples taken using a chosen interval distance or spacing. The choice of sampling sites is mot based on choices made using observable differences in the study area. The distance between sampling sites should be selected to detect variable data without collecting more data than necessary. This can be determined with a preliminary study.
Scientific methodologies:
Sample timing?
-To ensure data variability is detected.
-Selection of time intervals between samples.
Sample timing: If the factor that is being measured changes with time, then it will be necessary to sample on different occasions to produce a mean result that represents the ‘typical’ value or to identify trends in changing values.
The ideal time intervals between samples depends upon the the rate at which values change. This can be determined with a preliminary study.
What are some examples of time scales and variables that may be affected?
timescale variable
Long-term changes -population changes,
e.g. tigers
-Area of rainforest
-atmospheric CO2 level
Seasonal changes -presence of migratory
species
-population of annual
wildflower
-temperature
-atmospheric CO2 levels
-river level
Diurnal changes (24 hours) -light levels
-temperature
-atmospheric CO2 levels
Weather-related changes(mins-weeks)
-flying insect activity
- wind velocity
- temperature
Other short-term changes -road noise levels
Scientific methodologies:
Sample size?
Dependent on sample homogeneity.
If the variable being measured is not homogenous (the same) then larger samples are more likely to produce representative results. The sample size needed can be determined with a preliminary study.
Scientific methodologies:
Number of samples?
-Dependent on data variability.
-To enable analysis of statistical significance.
A single sample may not be representative as there may be variability between samples. Collecting multiple samples will help to eliminate the effect of variability. The number of samples needed to produce a representative mean depends upon the degree of scatter around the mean. This can be found using a preliminary study.
The degree of scatter of values around the mean can be assessed by calculating the standard deviation.
The number of samples collected will also depend upon how the results will be analysed. It is easier to assess the degree of statistical significance of the results with larger numbers of samples.
Scientific methodologies:
Standardisation of techniques?
To allow comparisons between different studies/ensure consistent reliability.
To allow comparison of the results that are collected at different times, in different locations, or by different researchers, they must all be collected in exactly the same way.
Other variables that may affect the results must either be controlled or monitored to assess their affects.
Scientific methodologies:
Collection of statistically significant data?
Experimental design should allow the assessment of statistical significance of the data collected.
Sampling techniques:
Methods:
quadrats- quadrat size selection?
Lichens 10cm x 10cm
Ground flora 0.5m x 0.5m
Trees in a forest 100m x 100m
Bird survey (presence, not population census) 10km x 10km
Sampling techniques:
Methods:
types of quadrat?
open frame quadrat
grid quadrat
point quadrat
Sampling techniques:
Methods:
types of quadrat- what is an open frame quadrat?
Open frame quadrat
These just mark out a study area. Smaller ones may be made of metal or plastic frame. Larger ones use reference coordinates marked out by measuring tapes or GPS coordinates.
Sampling techniques:
Methods:
types of quadrat- what is a grid quadrat quadrat?
These are sub-divided into smaller squares, for example, 10 x 10, each small square representing 1% of the area.
Sampling techniques:
Methods:
types of quadrat- what is a point quadrat quadrat?
These are used to collect similar information to other quadrats but they do not use a square area. They can be used where ground flora is too tall for other quadrat types because it would be flattened and provide unrepresentative results: a percentage cover estimate would be inaccurate and shorter plants may be obscured by the flattened foliage of taller plants. A wooden frame supports metal rods, usually 10. Each time a rod touches a leaf it counts as 10% cover.
What are the limitations of quadrats?
Limitations
Subjective judgement may be involved e.g. estimating percentage cover.
The quadrat frame may flatten or move plants
Sampling techniques:
Methods:
what does Kick sampling collect?
Kick sampling collects invertebrates that live on riverbeds.
Sampling techniques:
Methods:
kick sampling method?
Method
A net is held with its straight edge on the riverbed and its face open to the current.
The riverbed in front of the net is disturbed by repeatedly scraping a boot over it. Invertebrates that are dislodged are caught in the net. Controlling the number, duration, and length of kicks can be used to standardise the method.
Sampling techniques:
Methods:
kick sampling limitations?
Limitations
-Accurate standardisation is difficult.
-Buried organisms or those fixed on stones may not be dislodged.
-Some organisms can swim and avoid the net.
-The water flow rate is variable and affects capture rate.
-Fragile organisms may be destroyed.
Sampling techniques:
Methods:
what do surber samplers provide?
Surber sampling provides a more standardised technique than kick sampling.
Sampling techniques:
Methods:
surber sampler method?
Method
A fixed area of the riverbed is sampled within a box-like frame.
The riverbed is disturbed using a trowel or a similar tool and stones are inspected manually.
Netting at the sides of the opening reduces the number of organisms that escape.
Sampling techniques:
Methods:
surber sampler limitations?
Limitations
They can only be used where the water flow is fast enough to carry organisms into the net.
The small sample size can make it difficult to set on rough substrates in deep water. Can lead to the loss
Sampling techniques:
Methods:
colonisation media method?
Method
A method of demonstrating the presence of species by providing places that they can colonise which can then be checked. Some species can be monitored by providing suitable habitats that they may colonise.
Sampling techniques:
Methods:
colonisation media limitations?
Limitations
Not all species can be monitored as they may not use the media.
Accurate population estimates are not possible as the proportion that colonises the media is not known.
Sampling techniques:
Methods:
what do pitfall traps sample?
Pitfall traps are used to sample populations of mobile animals on the ground surface.
Sampling techniques:
Methods:
Pitfall traps methods?
Method
A container is placed in a hole in the ground with its open-end making a close fit with the soil and the top being level with the ground surface.
A cover is supported over the opening to exclude predators that may eat animals that are caught and to prevent flooding by rain.
The trap is checked after a standard period of time, e.g. 24 hours.
A preservation fluid may be used to kill captured animals for later study.