Research methods Flashcards

(124 cards)

1
Q

Scientific methodologies:
Sample location: random sampling?

A

Importance of the avoidance of bias.

Random sampling: if the study area has a regular shape then a grid can be laid out and the coordinates of sampling sites selected using random numbers. If the study area has an irregular shape then all the possible sampling sites can be numbered and selected using a random number generator.

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2
Q

Scientific methodologies:
Sample location: systematic sampling?

A

Regular sample intervals.

Transects – applied to ‘environmental gradients’:

line transects
belt transects
continuous/interrupted transects.

systematic sampling: this involves samples taken using a chosen interval distance or spacing. The choice of sampling sites is mot based on choices made using observable differences in the study area. The distance between sampling sites should be selected to detect variable data without collecting more data than necessary. This can be determined with a preliminary study.

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3
Q

Scientific methodologies:
Sample timing?

A

-To ensure data variability is detected.
-Selection of time intervals between samples.

Sample timing: If the factor that is being measured changes with time, then it will be necessary to sample on different occasions to produce a mean result that represents the ‘typical’ value or to identify trends in changing values.
The ideal time intervals between samples depends upon the the rate at which values change. This can be determined with a preliminary study.

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4
Q

What are some examples of time scales and variables that may be affected?

A

timescale variable
Long-term changes -population changes,
e.g. tigers
-Area of rainforest
-atmospheric CO2 level
Seasonal changes -presence of migratory
species
-population of annual
wildflower
-temperature
-atmospheric CO2 levels
-river level
Diurnal changes (24 hours) -light levels
-temperature
-atmospheric CO2 levels
Weather-related changes(mins-weeks)
-flying insect activity
- wind velocity
- temperature
Other short-term changes -road noise levels

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5
Q

Scientific methodologies:
Sample size?

A

Dependent on sample homogeneity.

If the variable being measured is not homogenous (the same) then larger samples are more likely to produce representative results. The sample size needed can be determined with a preliminary study.

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6
Q

Scientific methodologies:
Number of samples?

A

-Dependent on data variability.
-To enable analysis of statistical significance.

A single sample may not be representative as there may be variability between samples. Collecting multiple samples will help to eliminate the effect of variability. The number of samples needed to produce a representative mean depends upon the degree of scatter around the mean. This can be found using a preliminary study.

The degree of scatter of values around the mean can be assessed by calculating the standard deviation.
The number of samples collected will also depend upon how the results will be analysed. It is easier to assess the degree of statistical significance of the results with larger numbers of samples.

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7
Q

Scientific methodologies:
Standardisation of techniques?

A

To allow comparisons between different studies/ensure consistent reliability.

To allow comparison of the results that are collected at different times, in different locations, or by different researchers, they must all be collected in exactly the same way.
Other variables that may affect the results must either be controlled or monitored to assess their affects.

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8
Q

Scientific methodologies:
Collection of statistically significant data?

A

Experimental design should allow the assessment of statistical significance of the data collected.

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9
Q

Sampling techniques:
Methods:
quadrats- quadrat size selection?

A

Lichens 10cm x 10cm
Ground flora 0.5m x 0.5m
Trees in a forest 100m x 100m
Bird survey (presence, not population census) 10km x 10km

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10
Q

Sampling techniques:
Methods:
types of quadrat?

A

open frame quadrat
grid quadrat
point quadrat

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11
Q

Sampling techniques:
Methods:
types of quadrat- what is an open frame quadrat?

A

Open frame quadrat
These just mark out a study area. Smaller ones may be made of metal or plastic frame. Larger ones use reference coordinates marked out by measuring tapes or GPS coordinates.

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12
Q

Sampling techniques:
Methods:
types of quadrat- what is a grid quadrat quadrat?

A

These are sub-divided into smaller squares, for example, 10 x 10, each small square representing 1% of the area.

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13
Q

Sampling techniques:
Methods:
types of quadrat- what is a point quadrat quadrat?

A

These are used to collect similar information to other quadrats but they do not use a square area. They can be used where ground flora is too tall for other quadrat types because it would be flattened and provide unrepresentative results: a percentage cover estimate would be inaccurate and shorter plants may be obscured by the flattened foliage of taller plants. A wooden frame supports metal rods, usually 10. Each time a rod touches a leaf it counts as 10% cover.

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14
Q

What are the limitations of quadrats?

A

Limitations
Subjective judgement may be involved e.g. estimating percentage cover.
The quadrat frame may flatten or move plants

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15
Q

Sampling techniques:
Methods:
what does Kick sampling collect?

A

Kick sampling collects invertebrates that live on riverbeds.

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16
Q

Sampling techniques:
Methods:
kick sampling method?

A

Method
A net is held with its straight edge on the riverbed and its face open to the current.
The riverbed in front of the net is disturbed by repeatedly scraping a boot over it. Invertebrates that are dislodged are caught in the net. Controlling the number, duration, and length of kicks can be used to standardise the method.

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17
Q

Sampling techniques:
Methods:
kick sampling limitations?

A

Limitations
-Accurate standardisation is difficult.
-Buried organisms or those fixed on stones may not be dislodged.
-Some organisms can swim and avoid the net.
-The water flow rate is variable and affects capture rate.
-Fragile organisms may be destroyed.

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18
Q

Sampling techniques:
Methods:
what do surber samplers provide?

A

Surber sampling provides a more standardised technique than kick sampling.

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19
Q

Sampling techniques:
Methods:
surber sampler method?

A

Method
A fixed area of the riverbed is sampled within a box-like frame.
The riverbed is disturbed using a trowel or a similar tool and stones are inspected manually.
Netting at the sides of the opening reduces the number of organisms that escape.

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20
Q

Sampling techniques:
Methods:
surber sampler limitations?

A

Limitations
They can only be used where the water flow is fast enough to carry organisms into the net.
The small sample size can make it difficult to set on rough substrates in deep water. Can lead to the loss

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21
Q

Sampling techniques:
Methods:
colonisation media method?

A

Method
A method of demonstrating the presence of species by providing places that they can colonise which can then be checked. Some species can be monitored by providing suitable habitats that they may colonise.

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22
Q

Sampling techniques:
Methods:
colonisation media limitations?

A

Limitations
Not all species can be monitored as they may not use the media.
Accurate population estimates are not possible as the proportion that colonises the media is not known.

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23
Q

Sampling techniques:
Methods:
what do pitfall traps sample?

A

Pitfall traps are used to sample populations of mobile animals on the ground surface.

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24
Q

Sampling techniques:
Methods:
Pitfall traps methods?

A

Method
A container is placed in a hole in the ground with its open-end making a close fit with the soil and the top being level with the ground surface.
A cover is supported over the opening to exclude predators that may eat animals that are caught and to prevent flooding by rain.
The trap is checked after a standard period of time, e.g. 24 hours.
A preservation fluid may be used to kill captured animals for later study.

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25
Sampling techniques: Methods: Pitfall traps limitations?
Limitations Only mobile animals that live on the ground surface are caught, meaning more mobile species are more likely to be caught. Trapped carnivorous animals may kill other organisms that have been caught. Some species avoid traps or can escape. If a preservative is used, it may attract or repel certain species.
26
Sampling techniques: Methods: sweep nets methods?
Method Sweep nets are sturdy nets used to sample invertebrates in ground vegetation such as grassland. The net is swept through the vegetation so that organisms that are dislodged are caught. Standardisation of the method can be attempted by using the same number of sweeps of the same length, for a set time.
27
Sampling techniques: Methods: sweep nets limitations?
Limitations Mobile species may escape, such as flying insects.
28
Sampling techniques: Methods: what are beating trays used to sample?
Beating trays are used to sample invertebrates present on vegetation above the ground.
29
Sampling techniques: Methods: beating trays methods?
Method A sheet or tray is placed beneath the vegetation which is beaten several times with a stick. The invertebrates that are dislodged fall onto the sheet and are collected for identification and counting.
30
Sampling techniques: Methods: beating trays limitations?
Limitations It is difficult to standardise the method between researchers e.g. the vigour of hitting the branches. Some species are not dislodged. Thick branches shake less, so organisms are less likely to be dislodged. Some species fly away and are not caught. Higher branches cannot be reached.
31
Sampling techniques: Methods: what are light traps used for?
Light traps are used to collect night-flying insects that are attracted by bright lights.
32
Sampling techniques: Methods: Light traps methods?
Method Flying insects, especially moths that are attracted to the light fall into a container. The container is checked the following morning. The insects that have been caught are identified and counted.
33
Sampling techniques: Methods: Light traps limitations?
Limitations Moths can only fly during their adult phase and activity is affected by the weather and seasons, so the failure to find a species does not mean it is absent. The brightness and light wavelengths emitted by the light can affect their attractiveness to insects, so it may be difficult to compare results produced by different types of traps.
34
Sampling techniques: Methods: what are Tüllgren funnels used to sample?
Tullgren funnels are used to sample mobile invertebrates in leaf litter or the soil.
35
Sampling techniques: Methods: Tüllgren funnels methods?
Method A sample of leaf litter or soil is placed on a mat beneath a light. The light and heat repel some mobile invertebrates which move downwards, pass through the mesh and fall into a container. The Tullgren funnel is left operating for a standard period of time. The invertebrates collected are identified and counted.
36
Sampling techniques: Methods: Tüllgren funnels limitations?
Limitations Only some species that are repelled by heat or light will be collected. Some organisms may die or stop moving due to the heating or desiccation before they are collected. Some organisms are too large to pass through the mesh.
37
Sampling techniques: Methods: why do we use extraction of earthworms from soil?
Earthworms are difficult to observe, so they must be removed from soil for their populations to be monitored.
38
Sampling techniques: Methods: extraction of earthworms from soil methods?
Method Mark out a sample area of a suitable size 0.5m x 0.5m. Remove vegetation that may hide worms at the surface. Add an irritant solution e.g. detergent or mustard in water. Keep adding until the soil is saturated..Saturate the surrounding area so worms don’t move sideways. Add more as the solution infiltrates into the soil. Collect worms that come to the surface. Continue collecting until no more worms appear / for suitable time / standardised time period. Count / measure / weigh / identify the collected worms.
39
Sampling techniques: Methods: extraction of earthworms from soil limitations?
Limitations Irritant solutions may not percolate through impermeable soil. Irritating solutions may not reach the deepest worms. Worms may move sideways or down. Worms may not move / Worms may move too slowly to be collected during the study period Worms may die.
40
Quantitative/comparative/numerical measures: abundance scales, eg DAFOR scales
Abundance How much of a species there is. Use the DAFOR scale to judge abundance. Is subjective, not quantitative (Dominant, abundant, frequent, occasional and rare).
41
Quantitative/comparative/numerical measures: species richness?
Species richness Number of different species in an area.
42
Quantitative/comparative/numerical measures: species diversity?
Species diversity is measured using diversity indices, which quantify the relationship between the number of different species and the abundance of individuals within each species
43
Quantitative/comparative/numerical measures: species frequency?
Species frequency How dispersed a species is. Done by measuring the proportion of that species per sample.
44
Quantitative/comparative/numerical measures: species density?
Species density Number of species per area.
45
Quantitative/comparative/numerical measures: percentage cover?
Percentage cover of plants Judge ground for ground flora and sky for tree cover.
46
Quantitative/comparative/numerical measures: Lincoln Index method?
However for animals you can estimate population using mark-capture-release method and the Lincoln index: Catch a sample, using a named technique and count them. Then mark them (shave fur, attach a ring on birds’ leg, tag ear and microchip). Release them. A set time later captures a second sample - count the total end count how many had been marked already.
47
Quantitative/comparative/numerical measures: Lincoln Index calculation?
Then use the Lincoln index - Must need a large sample for validity. Estimated population = 1st sample total x 2nd total Those marked in 2nd sample
48
Quantitative/comparative/numerical measures: what assumptions does the Lincoln Index depend on?
This estimate relies on assumptions: -All individuals have the same chance of getting caught. -Marked individuals move freely within the population. -Marks do not fall off. -Marking doesn’t affect survival rate. -Population hasn’t changed in size since the 1st sample. -Animals are mobile and can be caught.
49
Quantitative/comparative/numerical measures: Simpson’s Index of Biodiversity equation?
Assessing Biodiversity We can assess the variety and abundance of species in an area as well as their evenness (how comparable different frequencies are). D = diversity N = total number of all species n = Total number of one species ∑ = sum of all n (n - 1) D= N(N-1) --------- Σn(n-1) A higher value for D = higher biodiversity. The more stable an ecosystem is.
50
Measurement of abiotic factors: light intensity?
A calibrated light meter can be used to measure light intensity. The method must be standardised, especially the sensor orientation.
51
Measurement of abiotic factors: Temperature?
A thermometer.
52
Measurement of abiotic factors: wind velocity? How is it standardised?
This is normally measure with an electronic anemometer. The use of an anemometer must be standardised in the following ways: - distance from the ground - no obstacles to air flow - axis of rotation must be horizontal (impeller type) or vertical (rotating cup type) - impeller type anemometers must face into the wind
53
Measurement of abiotic factors: what are the two methods to measure humidity?
1 - whirling hygrometer 2- electronic humidity meter
54
Measurement of abiotic factors: what is a whirling hygrometer?
This is the traditional method of measuring humidity. This uses two thermometers, one being dry while the other is kept wet. When they are spun, the wet bulb is cooled by the evaporation of the water, the dry bulb is not cooled. The rate of evaporation on the wet bulb depends on the humidity, so the temperature difference between the two thermometers can be compared with a table of values to estimate the humidity. To get an accurate reading, the hygrometer must be spun until there is no further cooling.
55
Measurement of abiotic factors: what is a electronic humidity meter?
As with all electronic meters, they must be calibrated to ensure that the readings are accurate
56
Measurement of abiotic factors: what is the purpose of measuring water turbidity?
- to estimate the turbidity of water caused by suspended solid particles
57
Measurement of abiotic factors: what are the two methods of measuring water turbidity?
- secchi disc - turbidity bottle
58
Measurement of abiotic factors: method of measuring water turbidity- Secchi disc?
- lower a secchi disc into the water until the black and white sectors cannot be distinguished - record the depth of the disc (length of string below the water level)
59
Measurement of abiotic factors: method of measuring water turbidity- Secchi disc limitations?
- the depth judgement is subjective - sunlight levels may affect visibility - the water may not be deep enough for the segments to become indistinguishable
60
Measurement of abiotic factors: method of measuring water turbidity- turbidity bottle?
- add a standard depth of water to a turbidity bottle - the hatched circles at the bottom of the bottle are observed to see which are obscured - note the coarsest circle which cannot be distinguished
61
Measurement of abiotic factors: method of measuring water turbidity- turbidity bottle limitations?
- judging which circles can be distinguished is subjective - light levels may affect visibility
62
Measurement of abiotic factors: method of measuring water pH?
pH Meter: A device that measures the acidity or alkalinity of a solution by detecting the concentration of hydrogen ions (H+) in the water. pH Paper: A piece of paper that contains a mixture of indicators, which change colour when exposed to different pH levels, allowing for a visual indication of the acidity or alkalinity.
63
Measurement of abiotic factors: what are the three methods of measuring water ion concentration, eg nitrates?
A wide range of methods is used to measure the concentration of dissolved ions. Most methods use colourimetry where the extent of a colour change involving the ions to be measured is used to estimate the ion concentration. test strips electronic colourimeter ion selective electrodes
64
Measurement of abiotic factors: what are the three methods of measuring water ion concentration, eg nitrates- test strips?
the test strips have bands of the reagents that change colour when they react with the ion to be measured the strips are dipped into the solutions being analysed then compared with a reference colour chart.
65
Measurement of abiotic factors: what are the three methods of measuring water ion concentration, eg nitrates- electronic colourimeter?
A reagent is added to the test solution. The intensity of the colour produced is measured by a light meter that is adjusted to measure specific wavelength of light.
66
Measurement of abiotic factors: what are the three methods of measuring water ion concentration, eg nitrates- ion selective electrodes?
An electronic probe is put into the test solution. The electrode potential that is produced by the solution between two electrodes is used as a measure of the ion concentration.
67
Measurement of abiotic factors: what are the three methods of measuring water ion concentration, eg nitrates- limitations?
the presence of other ions can lead to inaccurate results
68
Soil analysis: what is soil texture?
soil texture assesses the proportions of the mineral particles in different size categories: sand, silt, and clay. Two methods used to measure the proportions of sand, silt, and clay. component particle diameter range/ mm sand > 0.02-2 silt 0.002-0.02 clay <0.002
69
Soil analysis: what are the two methods to measure soil texture?
-soil sieves -sedimentations
70
Soil analysis: methods to measure soil texture- soil sieves?
- the stack of sieves is assembled in order of mesh size, with the coarsest mesh at the top - a dried, crushed soil sample is placed in the top sieve - the sieve stack is shaken by hand, or mechanically, for two minutes (long enough for no more particles to fall through). The particles drop through the sieves until they reach a sieve where holes are too small to pass through - each portion is weighed and the % composition of the three portions calculated - wet sieving is also possible. The sub-samples collected in each sieve must be dried before weighing . the top sieve (mesh size > 2mm) contains pebbles, twigs etc. These are not part of the soil. . the second sieve (0.02-2mm) contains sand. (some stacks have an extra sieve to separate coarse and fine sand) . the third sieve (0.002-0.02mm) contains silt . the base container holds clay
71
Soil analysis: methods to measure soil texture- sedimentation?
- larger objects are removed from a dried soil sample by using a 2mm sieve, or by hand, for example, pebbles, twigs - the soil is crushed to ensure the particles are separated - a measuring cylinder is about half filled with soil, then topped up with water - the top is sealed then the cylinder is shaken by repeatedly inverting it - the suspension is allowed to settle - the total depth of the settled soil components is measured after 2 minutes, 2 hours, and 2 days - the proportion of the total volume of each textual category can then be calculated
72
Soil analysis: what are the methods used to measure pH?
- colourimetry → with universal indicator solution → with universal indicator paper - electronic pH meters
73
soil analysis: colourimetry method with universal indicator solution?
Universal indicator is a mixture of pH indicators that show characteristic colour changes over the pH range of 0 to 14. It can be used as a solution or on pH test papers. Universal indicator solution- pH indicator chemicals absorb different wavelengths of light at different pHs. This colour change is predictable and can be used to measure pH by comparing the colour of the indicator with a reference chart. This method is simple but is subjective because it relies upon the judgement of colour. A standard colourimetric method can be used to estimate the pH of a soil sample. . add 2.5 cm³ of soil to a test tube . add 1cm³ of barium sulfate (toxic), which helps the soil particles to settle . add 3.5 cm³ distilled water . add 10 drops of universal indicator solution - firmly push a bung into the test tube . shake thoroughly and leave to stand for 15 minutes . compare the colour of the coloured solution above the soil with a colour chart
74
soil analysis: colourimetry method with universal indicator papers?
these are strips of absorbent paper that are impregnated with universal indicator solution. The papers can be dipped into the solutions to be tested and any colour changes compared with a reference chart. - for soil samples, the procedure is similar to that outlined for universal indicator solution. No universal indicator solution is added. A universal indicator paper is dipped into the settles water sample and the colour change is compared with a colour chart. - for water samples the papers are simply dipped into the sample and compared with a colour chart
75
soil analysis: what are the limitations with colourimetry method with universal indicator papers and colourimetry method with universal indicator solution?
using colour comparison charts is subjective
76
Soil analysis: method of electronic pH meters? What do we have to ensure when collecting our data?
The quantitative values produced by electronic meters can be easy to interpret and compare but it is important to ensure the results area accurate, representative, and collected using a standardised method. - the pH meter should be calibrated using buffer solutions of known pH - the meter should be left to stabilise for long enough to give stable readings - the probe should be inserted to a standard depth
77
Soil analysis: method of electronic pH meters - limitations?
- the need to regularly calibrate the probe with buffer solutions makes this method less convenient than universal indicator papers
78
Soil analysis: method of estimating the soil water content?
The water content of a soil can be estimated by noting the mass drop of a weighed soil sample when the water it contains is evaporated by heating. Method: 1- The soil sample is collected using a standardised technique such as a core sampler or auger. 2- The soil sample is placed in a sealed bag to prevent water loss by evaporation. 3- Larger mineral particles, organic detritus and visible organisms are removed. 4- The soil sample is placed in a pre-weighed evaporating basin or similar container. 5- The basin is heated at about 100°C for 24 hours. 6- The basin is reweighed. 7- stages 5) and 6) are repeated until a constant mass is reached. The percentage water content can then be calculated
79
Soil analysis: The percentage water content can then be calculated - what is the equation?
percentage water content= wet soil mass- dry soil mass ---------------------------------------- x 100 wet soil mass
80
Soil analysis: Method for estimating the soil organic matter content?
The organic matter content of a soil can be estimated by heating the dry soil to burn off the organic matter. Method: (follow steps 1-7 of the soil water content method) then (as you then have a completely dry sample): 8- A dry soil sample is placed in a pre-weighed crucible which is reweighed. 9- The crucible is heated strongly in a furnace or with a Bunsen burner. 10- The crucible is re-weighed. If the heating temperature is above 550°C then minerals may break down, for example, calcium carbonate breaking down, releasing carbon dioxide. This would give an inaccurately high estimate of organic matter as the reduction in mass would not be caused by the loss of organic matter alone. 11- Stages 9) and 10) are repeated until a constant mass is reached. The percentage organic matter content can then be calculated.
81
Soil analysis: The percentage organic matter content can then be calculated - what is the equation?
percentage organic matter content= Dry soil mass - Burnt soil mass -------------------------------------------- x 100 Dry soil mass
82
Soil analysis: How do we measure soil bulk density?
Soil bulk density is the mass of dry soil per unit volume. The volume of a soil sample can be controlled by using a soil auger of known cross-sectional area and pushing it into the soil at a chosen depth. The dry soil mass can be found by weighing the soil after it has been heated as about 100°C to constant weight.
83
Soil analysis: The use of a soil triangle?
A soil triangle is a triangular graph used to display the combined proportions of the three minerals soil components. Different areas of the graph represent soils with characteristic properties.
84
Fieldwork and laboratory activities?
Fieldwork and laboratory activities These should include, but not limited to, the following. Ecological studies in suitable available habitats: - population size/ density - species frequency - species distribution - biodiversity - soil analysis -The effect of climatic variability on the use of renewable energy resources: insolation intensity, wind velocity. - Factors affecting the rate of heat loss: insolation, volume. - The use of biotic indices in monitoring pollution: lichens, aquatic invertebrates. - The effect of pH on seed germination. - The effect of water turbidity on light penetration. - The effect of inorganic nutrients on the growth of aquatic plants/ algae. - Factors affecting noise levels: distance from source, acoustic insolation. - The effect of slope and vegetation on rain splash erosion. - The effect of trees on microclimates.
85
Population studies: Why are ecological monitoring surveys important?
Ecological monitoring is a very important part of wildlife conservation programmes, Good surveys are essential for many reasons: - to find out which species are present - to monitor the habitat conditions, how they change, and which conditions individual species require - to monitor features of populations and how they change over time such as: . population size . age structure . growth rates . breeding rates . territory size . population movements . reliable, representative data are important in making good conservation management decisions There are many different techniques that are used to monitor ecological conditions in ecosystems and the species that live there. The specific technique that is appropriate depends upon what is being measured. It is also affected by the rapid development of new technologies that may produce better results: more representative, quicker, more cost effective.
86
Specialist techniques used in ecological research: Photography/ imagery- what are the 2 spec methods 1 off spec method?
A rapidly expanding range of imagery systems is available to collect environmental info. spec - motion sensitive cameras . these can be used for habitat monitoring to detect the Prescence or activity of animals, using visible light photography during the day or infrared photography at night. - databases of physical features may be used to identify individuals e.g. tiger stripe patterns, whale shark spots, whale and dolphin fin damage. (image databases) . the individuals of some species can be recognised from image databases of unique features, for example, tiger facial stripes, cheetah tail markings, whale shark spot patterns, dolphin fin marks and injuries, zebra stripes off-spec - closed circuit TV (CCTV) . CCTV cameras can be used to observe animal behaviour with a lower risk of disturbance than if researchers visit the site, for example, for monitoring birds' nests.
87
Specialist techniques used in ecological research: Marking: tags, rings, collars etc.?
A range of methods is used to identify individual animals that have been previously caught, for example, rings placed on bird's legs provide information on movements and lifespan if they are caught later.
88
Specialist techniques used in ecological research: Auditory monitoring (sounds)/ sonograms: birds, bats, cetaceans?
Some species that cannot be seen can be detected by the sounds they produce. Sonograms: Sonograms are records of the sounds made by taxa such as dolphins, bats, and some insects. They can be used as evidence of their presence, abundance and activity.
89
Specialist techniques used in ecological research: Radio/ GPS/ satellite tracking (position monitoring)?
A range of technologies can be used to monitor the location of individuals. - radio, GPS, and acoustic transmitters can be used to track animals in air or freshwater - in seawater, acoustic transmitters are used. They give real-time information of the current position of the animals carrying the transmitter. Geolocator tags may be used where transmitters would be too heavy, or where the battery life would be too short for a long-term study. They are small and only collect data on time and light. When the animal is re-caught, the data can be downloaded and used to work out the latitude and longitude of the animals for each day during the study. Geolocator tags are used to track the movements of birds that are too small to carry radio transmitters. The data on location can be downloaded if they are recaptured.
90
What data can be collected by satellite sensors?
- to monitor habitat changes, - water availability, - rock density, - ice cover, - ice thickness,
91
what may databases contain?
blood, tissue samples, DNA, eDNA
92
what does indirect evidence show?
It shows the Prescence of the species even if it is not actually seen
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What are some examples of indirect evidence?
- nest/ burrows - droppings - can give information on diet, gender, territories - feeding marks, eg nuts, fruit - owl pellets - also give information on diet - tracks/ footprints - territorial marks, eg scratching posts
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The effects of climatic variability on the use of renewable energy resources: insolation intensity.
light intensity can be measured using an electronic light meter. As with all electronic meters, this should be calibrated to ensure it is reliable and can be used with a standardised technique. Factors that affect the intensity of solar power include the time of day, time of year, and cloud cover.
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The effects of climatic variability on the use of renewable energy resources: Wind velocity.
The ideal location for a wind farm is usually where the wind velocity is reliably high. The power available is not proportional to mean wind velocity as doubling wind velocity increases available power eight-fold (8x as great). The effect of local factors on wind velocity can be investigated: - distance from the ground - topography - aspect in relation to prevailing winds - nearby trees or buildings
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Factors affecting the rate of heat loss: insulation?
The heat energy that must be added to a building, furnace, or heat store to maintain a constant temperature depends upon the rate at which heat is lost. Reducing the rate at which heat is lost will therefore reduce the rate at which heat must be added. - thermal insulation: The rate of heat loss depends upon the thermal conductivity of the surrounding material. The thicker the surrounding material, and the lower the thermal conductivity, the lower the rate of heat loss, but each additional layer has a smaller effect than the previous ones. If the value of the heat saving and the cost of the insulation are known then the optimum thickness of insulation can be calculated The estimation of heat loss requires careful planning and interpretation. The total amount of heat energy stored can be calculated using the specific heat capacity and temperature of the materials used. However, the temperature will vary throughout the volume, being lower near the surfaces and higher in the core. It may also be higher near the top, if water-filled containers are used where convection currents may occur.
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Factors affecting the rate of heat loss: volume? What needs to be standardised?
The heat energy that must be added to a building, furnace, or heat store to maintain a constant temperature depends upon the rate at which heat is lost. Reducing the rate at which heat is lost will therefore reduce the rate at which heat must be added. - Volume: The heat energy stored in a hot mass of material is lost through the external surface. As the volume increases the surface area to volume ratio changes so that the rate of heat loss also changes. The total amount of heat loss will be increased because the surface area and amount of heat stored have also gone up. However, the rate of heat loss per unit volume goes down so the temperature drops more slowly. The principles of the effect of volume on the rate of heat loss can be investigated with a range of objects of different volumes. Factors that should be standardised: - the material the objects are made of, for example, clay cubes, water-filled round-bottomed flasks, or PET bottles, - 3D shape, - colour, darkness, and texture of the surface, - experimental conditions such as room temperature, air movements, and contact with other materials and surfaces, The estimation of heat loss requires careful planning and interpretation. The total amount of heat energy stored can be calculated using the specific heat capacity and temperature of the materials used. However, the temperature will vary throughout the volume, being lower near the surfaces and higher in the core. It may also be higher near the top, if water-filled containers are used where convection currents may occur.
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Use of biotic indices in monitoring pollution. How do biotic indices show this?
A biotic index is a method of monitoring environmental conditions, usually pollutants, by the presence/ absence, state of health/ growth, and abundance/ diversity of species which would normally be present.
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Use of biotic indices in monitoring pollution. What are the advantages of using biotic indices?
- they monitor current pollution levels and can be used to estimate past levels. - they can help trace the source of the pollution. - they can be very sensitive to small changes. -Very low levels of pollutants which bioaccumulate may be detectable in raised concentrations in organisms. - They do not need expensive monitoring equipment.
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Use of biotic indices in monitoring pollution. Disadvantages of using biotic indices?
- sorting samples can be time-consuming. - identification can be difficult for the inexperienced.
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Use of biotic indices in monitoring pollution. Features of good indicator species?
- easy to identify - normally present if there is no pollution - normally common if there is no pollution - widely distributed rather than found in groups - easy to find ideally there will be a range of species with different sensitivities to pollution. In this case they may disappear in an order which indicates the level of pollution
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Use of biotic indices in monitoring pollution. Atmospheric pollution and lichen biotic indices?
the long-term effects of pollutants can be difficult to assess by measuring the levels of the pollutants that cause the damage as these can fluctuate widely. For example, the atmospheric concentrations of sulfur dioxide and other acidic gases vary widely, mainly due to the varying rate of their removal by rain. This makes it difficult to measure the concentration of pollutants such that they can be reliably correlated to the damage they cause. A better indication of the pollution problems may be achieved by measuring the impact of the pollution through the abundance and diversity of the organisms that are affected. Organisms that are sensitive to pollution give an indication of past pollutant levels as well as what is happening now. Lichens are particularly sensitive to acidic conditions, with different species having different sensitivities so the presence or absence, size and state if health of different species can be used to produce a scale of the severity of pollution. This can be used to estimate long-term pollution conditions at different locations.
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Use of biotic indices in monitoring pollution. Atmospheric pollution and lichen biotic indices- what are the factors that should be standardised?
- the surface on which the lichens are found, as materials such as limestone or granite will also affect the pH, - the aspect of the surface in relation to the prevailing wind and therefore exposure to rain, - factors that influence lichen growth such as exposure to sunlight It is common to study lichen distribution on gravestones as their orientation normally faces west-east and the substrate can be standardised: they are often made of granite.
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Use of biotic indices in monitoring pollution. Aquatic pollution and aquatic invertebrate biotic indices.
A range of aquatic invertebrate taxa with different sensitivities to pollution are used to monitor pollution. They meet the desired criteria. The following taxa are often included: stonefly larvae, mayfly larvae, caddisfly larvae, freshwater shrimps, freshwater hoglouse, midge larvae
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The effect of pH on seed germination. Choice of plant species?
High and low pH conditions denature proteins and inactivate enzymes. Each species has its own range of tolerance. To investigate the effect of pH on germination, a number of choices must be made. - choice of plant species- some species make good experimental subjects: . small seeds . genetic uniformity . high normal germination rate . rapid germination These species are not necessarily the ones that produce results which can be applied directly to natural ecosystems
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The effect of pH on seed germination. Number of seeds and replicates?
-each test must use enough seeds to produce results that can be distinguished from each other if there is a real difference, but not so many than unnecessary extra work is undertaken. - each test must be replicated to allow anomalies to be identified and the results to be statistically valid
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The effect of pH on seed germination. pH range?
the range of pHs tested should cover the full range at which germination can take place. This can be established with a preliminary study using a range of widely spaced pHs. The pH range investigated in the final experiment would cover the range from the pH below the lowest pH at which germination occurred to the pH above the highest pH at which germination occurred.
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The effect of pH on seed germination. study duration?
the experiment should be continued until it is clear that seeds that have not germinated are not going to. The possibility that pH could delay germination must be eliminated.
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The effect of pH on seed germination. Other variables that should be standardised?
- temperature: the temperature should be standardised, ideally at a constant temperature that would occur during natural germination, - nutrient supplies: seeds do not need to absorb nutrients to germinates. They would only need to be provided if the plants were going to be grown beyond the time at which they would have exhausted the nutrients originally present in the seed, - using soil as a growth medium replicates the natural growth situation but may affect the experiment conditions, including pH,
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The effect of water turbidity on light penetration. what tests give semi0quantitative or qualitative results? what test gives quantitative results?
Measuring the turbidity of water is an important way of monitoring soil erosion. The turbidity of water can be measured using a number of techniques in the field or in the laboratory. Secchi discs and turbidity bottles give semi-quantitative or qualitative results. Electronic meters give quantitative data.
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The effect of water turbidity on light penetration. Electronic turbidimeters?
Most electric turbidimeters measure the amount of light that is scattered by the suspended particles in a water sample, usually at 90° to the angle at which it entered the water. An increase in turbidity causes more light to scatter, so the light reading rises. The light readings give relative values of the concentration of the suspended particles in the water sample. Quantitative estimates can be produced if a calibration curve is produced by measuring the light scatter from samples with known amounts of suspended matter. ( samples with higher turgidities cause more light scattering and give higher light readings )
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The effect of water turbidity on light penetration. Electronic turbidimeters- factors that must be standardised?
- brightness of the light bulb, - colour of light emitted by the lightbulb, - distance to the sample holder, - material used to produce the calibration samples, ( a dark room with no other light sources will produce more representative results )
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The effect of inorganic nutrients on the growth of aquatic plants/ algae. What is the general affect of leaching of nutrients?
The inorganic nutrients washed off fields or released in sewage effluent can increase the rate of growth of plants and algae and contribute to eutrophication.
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The effect of inorganic nutrients on the growth of aquatic plants/ algae. Measuring biomass growth?
The increase in growth caused by the availability of nutrients may cause an increase in biomass as more sunlight is converted into carbohydrates. Living matter is mainly water, so measuring living biomass is not necessarily a good indicator of the amount of carbohydrate present. It is especially difficult with aquatic plants where the surface water will affect the mass but drying it off may damage the plants. Dry biomass is a more precise measure but involves killing the plants or algae so long term studies cannot be carried out on the same organisms. If the increase in dry biomass is estimated on several occasions over a period of time then a large number of original samples will be needed since each is destroyed when it is weighed. For some aquatic plants, such as duckweed, leaf area can be used as a reasonable estimate of biomass and allows continued use of the same plants.
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The effect of inorganic nutrients on the growth of aquatic plants/ algae. Measuring biomass growth- other variables that must be controlled?
- temperature, - light levels, If nutrient solutions need to be topped up, it is best to transfer the plants to new solutions rather than adding more nutrients to the remaining nutrient solution which will have an unknown nutrient concentration.
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Factors affecting noise levels. How is it measured?
Noise levels can be measured with an electronic sound level meter but if one is unavailable many mobile phones incorporate sound level meters. If uncalibrated meters are used then the same one should be used to collect all the results. This will ensure that the degree of error is constant so they can be compared with each other.
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Factors affecting noise levels. Distance from source?
The fact that noise levels decline as the distance from the source increases is predictable. Collecting quantitative data and assessing variations in noise levels from sources such as roads and airports is more difficult. Because the decibel scale is logarithmic, a simple arithmetic mean does no give a true representation of the typical sound levels. The presence of sound absorbing or reflecting surfaces and topography can affect the noise levels, so the study sites should be as similar as possible.
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Factors affecting noise levels. Acoustic insolation?
This may be investigated under laboratory conditions with a source of sound, such as a speaker, surrounded by acoustic insulation of different materials or thickness. The effectiveness of insulation around roads or airports such as baffle mounds, embankments, walls, fences, or vegetation can be compared.
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The effect of slope and vegetation cover in rain splash erosion.
Scientific experimentation relies upon good experimental design which requires a knowledge of all the variables that could influence the results. Ideally, all the variables that are not being investigated should be standardised so that the only factor that effects the dependent variable (the results) is the independent variable that is being deliberately controlled. This is easier to do under laboratory conditions than under field conditions. Factors that cannot be standardised should be measured so that their influence on the results can be assessed.
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The effect of slope and vegetation cover in rain splash erosion. Variables that can affect rain splash erosion?
- rainfall intensity - soil texture - soil compaction - organic matter content - soil depth - permeability of the material beneath the soil - precipitation rate - volume per unit area per unit time - raindrop size - raindrop height drop - gradient - vegetation cover - root binding Many experimental procedures have limitations that are an unavoidable part of the method. It is important that these should be known and their impacts on the validity of the results appreciated and noted.
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The effect of trees on microclimates.
Trees affect the local climate in and around woodlands. Even individual trees produce microclimates. An understanding of how these microclimates are produced and the affects they have can alter management plans for urban areas and rural areas such as farmland, forestry plantations, and woodlands for wildlife conservation.
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The effect of trees on microclimates. Vegetation features?
- tree height - foliage characteristics - tree spacing - total area of woodland
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The effect of trees on microclimates. Features of the microclimate that can be measured?
- wind direction - wind velocity - light levels - humidity - soil moisture - temperature To measure the effect of trees on microclimates the general weather conditions must also be known to identify local differences
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Students must understand the general principles of scientific methodology and be able to apply these to a wide range of environmental situations and techniques.
- preliminary studies may be used to ensure the study will produce representative data - practical activities should be carried out with consideration of their environmental impacts and how these can be minimised.