Sequestering microRNA

Question 1

What are two ways to sequester miRNA

Answer 1

1. Have the ratio for the sponge RNA greater than the miRNA

2. Multiple binding sites for miRNA on the sponge RNA

Question 2

What are CircRNAs?

Answer 2

Circular RNAs used for sponging microRNAs

Question 3

Define CDR1as, and its role

Answer 3

• circular RNA having over 70 binding sites for miR-7

- major role is sequestering and transporting miR-7 in axons

Question 4

What is FISH?

Answer 4

• RNA fluourescence in situ hybridization

- A method that allows you to stain for multiple genes, each gene with a distinct fluorescence

Question 5

What two miRNAs does CDR1as have binding sites for?

Answer 5

miR-671 and miR-7

Question 6

What function does CDR1as play?

Answer 6

CDR1as and its direct
interactions with miRNAs are
important for sensorimotor gating
and synaptic transmission