Study Group Practical 1

Question 1

Inoculation Temperature

Answer 1

37(Degree Sign)C

Question 2

TSB

Answer 2

A nutrient-rich liquid medium used for cultivating bacteria.
B for BROTH

Question 3

TSA

Answer 3

A nutrient-rich solid medium used for growing bacterial cultures.

Question 4

TSB vs. TSA

Answer 4

TSB is liquid, used for growing bacteria in suspension; TSA is solid (contains agar), used for isolating colonies.

Question 5

Pure Culture

Answer 5

A culture containing only one type of microorganism.

Question 6

Mixed Culture

Answer 6

A culture containing two or more types of microorganisms.

Question 7

Contaminated Culture

Answer 7

A culture that contains unintended microorganisms.

Question 8

BAP

Answer 8

A type of enriched agar that contains red blood cells to support growth of fastidious bacteria and to observe hemolysis (breakdown of RBCs).

Question 9

Fastidious Bacteria

Answer 9

Bacteria that require specific nutrients or growth conditions.

Question 10

Solid Media

Answer 10

Culture media that is firm due to the presence of agar, used for isolating colonies.

Question 11

Semi-Solid Media

Answer 11

Culture media with a lower concentration of agar, used to test motility or support microaerophiles.

Question 12

Petri Plate

Answer 12

A shallow, flat, round dish used to hold solid culture media.

Question 13

Slant

Answer 13

A test tube containing solid media that has solidified at an angle, providing a larger surface area for growth.

Question 14

Deep

Answer 14

A test tube containing solid media that is fully solidified vertically, often used for anaerobic or motility tests.

Question 15

Agar Melting Temperature

Answer 15

100(degree sign)C

Question 16

Agar Holding Temperature

Answer 16

50(Degree sign)C

Question 17

Agar Pouring Temperature

Answer 17

45(Degree Sign)C

Question 18

Agar Resolidfication Temperature

Answer 18

40(Degree Sign)C

Question 19

Problems with pouring agar too cold

Answer 19

Lumps will occur in the plate or agar won’t pour

Question 20

Problems with pouring agar to hot

Answer 20

Condensation will occur in plate and many bacteria will be killed

Question 21

Why sterilize tubes before and after transfer

Answer 21

To prevent contamination from external microbes and ensure aseptic technique.

Question 22

How to sterilize tubes before and after transfer

Answer 22

Flame the opening of the tube using a Bunsen burner before and after transferring microorganisms.

Question 23

Why incubate upside down

Answer 23

To prevent condensation from dripping on agar surface and to read labels while being able to see organism from top.

Question 24

Asepsis

Answer 24

Practice of preventing contamination by microorganisms

Question 25

Where to start flaming loop?

Answer 25

Start at the base of the wire and move toward the tip.

Question 26

Why start loop sterilization at base

Answer 26

Complete sterilization of part of loop being used, not burning yourself. (Don't flame the handle!!)

Question 27

Possible contamination points in lab

Answer 27

Tube openings, loop tips, Petri dish lids, bench surfaces, gloves, and hands.

Question 28

Cover Slip

Answer 28

A small, thin piece of glass placed over a specimen on a slide for microscopy.

Question 29

Slide

Answer 29

flat piece of glass used to hold specimens for microscopic examination.

Question 30

Bunsen Burner

Answer 30

A device that produces a single open flame for sterilization or heating in the lab.

Question 31

Bilbious Paper

Answer 31

Absorbent paper used to blot excess liquid from microscope slides. (Can also be used to dry slides, but pat don't wipe!!)

Question 32

Striker

Answer 32

A tool used to ignite the Bunsen burner safely.

Question 33

Ubiquitous

Answer 33

Present or found everywhere (common in nature).

Question 34

Omnipresent

Answer 34

Present everywhere at the same time

Question 35

Ubiquitous vs. Omnipresent

Answer 35

“Ubiquitous” implies widespread presence; “omnipresent” implies being everywhere simultaneously, often in a figurative or absolute sense.

Question 36

Aseptic Technique

Answer 36

a set of practices in healthcare and other fields designed to prevent contamination by pathogenic microorganisms and reduce the risk of infection.