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Rachel Ingham
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Decks in this class (34)
History
What is genetic engineering,
When did scientists discover that...,
When did cohen and boyer pioneer ...
13
cards
DNA extraction
What is gene cloning,
Describe dna extraction
2
cards
Enzymes
What do nucleases do,
What do ligases do,
What do polymerases do
33
cards
Visualising DNA
What does gel electrophoresis do,
How does gel electrophoresis work,
How do you get a higher resolutio...
9
cards
PCR
What are the main components need...,
What is the general process,
How should primers be designed
8
cards
Vectors 1:Plasmids
How are clones typically generated,
What are plasmids,
What are the 2 main features of p...
12
cards
Vectors 2:Bacteriophages
How are complete bacteriophages f...,
Describe phage vectors,
What is the central stuffer
6
cards
Library construction
What is a genomic library,
How are cdna libraries made,
How is cdna made
3
cards
Library screening
What is the first step in screeni...,
What are the 2 different ways the...,
Explain how an amylase gene could...
8
cards
DNA sequencing
What is sequencing,
What is sanger sequencing,
What are dideoxyribonucleoside tr...
5
cards
Genomics
Whats in a genome
1
cards
Comparative genomics
What is comparative genomics,
What is metagenomics
2
cards
Synthetic genomics
What is synthetic genomics,
What can synthetic genomics be us...
2
cards
Functional genomics
What is functional genomics,
What are some methods in transcri...,
What are some methods in proteomics
6
cards
Structural proteomics
What is structural proteomics,
What are some techniques used
2
cards
Metabolomics
What is metabolomics used to study,
What are the 3 steps
2
cards
Metallomics
What is metallomics,
What are some methods used,
What is inductively coupled plasm...
3
cards
Pronuclear injection
What is pronuclear injection,
Describe the procedure,
What is in the dna that is injected
8
cards
Homologous recombination
What is hr,
What cells are used in hr,
Describe gene knockouts
9
cards
Commercial applications
Describe the first transgenic fish,
Describe canadian salmon,
Describe aquadvantage salmon
4
cards
Gene editing
What are zfns,
What 2 ways do zfns cut dna,
Describe the first zfn transgenic...
8
cards
Animal cloning
What is differentiation,
Describe dolly the sheep,
Describe xenotransplantation
5
cards
Recombinant protein expression
What is the point of recombinant ...,
Why use microbes,
What are the main uses for recomb...
3
cards
Detecting recombinant proteins
Describe electrophoresis,
Describe page,
Describe sds page
5
cards
Making an expression construct
What are some advantages to using...,
What are some disadvantages to us...,
Describe transcriptional fusion
8
cards
Perfect and problematic expression
Why is there a protein solubility...,
What cant e coli do to a protein,
What happens to the proteins prod...
5
cards
Yeast as an expression host
What are the 4 types of yeast exp...,
Describe yip,
Describe yep
8
cards
Plant transformation
What are some advantages of gm te...,
Why alter plants,
Describe stable transformation
17
cards
Biolistic transformation
Describe briefly particle bombard...,
What are the 7 steps in particle ...,
What are the advantages of partic...
9
cards
Protein engineering
What is a proteins secondary stru...,
Describe helices,
Describe strands sheets
13
cards
Alternate PCR
What can be changed in pcr,
What types of pcr change the temp...,
Describe colony pcr
18
cards
Enzyme selection
What are the 4 factors to choosin...,
Describe extension rate,
Describe fidelity
8
cards
Degenerate PCR
What are degenerate primers,
How are the primers designed,
What is codon bias
3
cards
Megaprimer based PCR
What is megaprimer pcr,
What novel sequences can be added,
Describe the primers used
3
cards
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BIO2030 Biotechnology
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