1
Q
What is a genomic library?
A
A collection of clones that include all of the DNA sequences of a given species that can be screened for any clones containing the sequence of interest
2
Q
How are cDNA libraries made?
A
From isolated mRNAs
3
Q
How is cDNA made?
A
Reverse transcriptase makes DNA from RNA
Linkers are added to the ends of the DNA to help it join to the vector
BIO2030 Biotechnology
flashcards
Decks in class (34)
# Cards
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History13
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DNA extraction2
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Enzymes33
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Visualising DNA9
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PCR8
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Vectors 1:Plasmids12
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Vectors 2:Bacteriophages6
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Library construction3
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Library screening8
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DNA sequencing5
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Genomics1
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Comparative genomics2
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Synthetic genomics2
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Functional genomics6
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Structural proteomics2
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Metabolomics2
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Metallomics3
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Pronuclear injection8
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Homologous recombination9
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Commercial applications4
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Gene editing8
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Animal cloning5
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Recombinant protein expression3
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Detecting recombinant proteins5
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Making an expression construct8
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Perfect and problematic expression5
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Yeast as an expression host8
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Plant transformation17
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Biolistic transformation9
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Protein engineering13
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Alternate PCR18
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Enzyme selection8
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Degenerate PCR3
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Megaprimer based PCR3
