Functional genomics

Question 1

What is functional genomics?

Answer 1

Understanding biological functions through the use of genomic/transcriptomic data
Investigation of cell functions
Assigns functions to genes

Question 2

What are some methods in transcriptomics?

Answer 2

Northern blots
in situ hybridisation 
RT-qPCR
digital droplet PCR
Microarray
RNA-seq

Question 3

What are some methods in proteomics?

Answer 3

2DE-MS
LC/MS
Metalloproteomics
Phosphoproteomics
Structural genomics

Question 4

What is the transcriptome?

Answer 4

The set of all the mRNA in a cell

Question 5

What are the discrepancies between protein and mRNA in cells?

Answer 5

mRNA levels do not always correlate with protein levels in a cell

Question 6

What are protein arrays used for?

Answer 6

Detection of biomarkers
Protein quantification in cell lysate
Protein: protein interactions
Antibody characterisation