additional info

Question 1

continuous synthesis of DNA

Answer 1

5-3 direction - leading strand

Question 2

discontinuous synthesis of DNA

Answer 2

3-5 direction - lagging strand made by okazaki fragments

Question 3

centrifuge process

Answer 3

• spun tube works based on centrifugal force
• lighter liquid supernatant drained off top, leaves heavier denser pellet of DNA forced to the bottom
• separated based on size, shape, density and viscosity

Question 4

why does the Q -PCR graph plateau?

Answer 4

run out of materials/ components to continue building
or amplification won’t add any more to it

• no more fluorescence (increase in y axis) because no more is being made but the number of cycles (x axis increase) is still happening

Question 5

difference in structure between ddNTPs and dNTPs

Answer 5

ddNTP DONT have an OH group on the 3rd carbon = cannot continue the building of the chain = termination