Probabilistic genotyping

Question 1

Continuous method of probabilistic genotyping

Answer 1

uses allele and peak height data considering biological parameters

Question 2

semi continuous method of probabilistic genotyping

Answer 2

uses allele and peak height data WITHOUT considering biological parameters

Question 3

Analytical threshold

Answer 3

determined from validation studies on equipment
distinguishes peak as allele or artefact
(i.e. if the height doesn’t pass the threshold then = artefact)

Question 4

Stochastic threshold

Answer 4

also determined from empirical data
any allele that sits above it = homozygous

Question 5

Clayton rules

Answer 5

1. identify - artefacts, alleles, contributors
2. determine - ratio of components
3. compare - profiles to reference samples
4. perform - statistical analysis