DNA extraction methods

Question 1

Solid Extraction

Answer 1

for low levels of DNA in sample (swabs or touch DNA)

Question 2

Epithelial extraction

Answer 2

high source samples (direct amount of skin/ bodily fluids)

Question 3

Differential extraction

Answer 3

for samples containing sperm –> washes are done to remove female cells

Question 4

Extraction negative

Answer 4

Used to identify contamination –> a blank sample is run

Question 5

Quantification of DNA

Answer 5

determining how much is present, using dyes or probes to track it
use inter calating dyes (fit into grooves w/in structure)

Question 6

types of quantifiers

Answer 6

1. quantifiler
2. Pico green - not human specific but quick and easy
3. HY - indicates how much male DNA is present