Where does DNA replication occur in eukaryotic cells?
Nucleus
Helicase
Unzipping enzyme that separates two strands of DNA, breaking thru hydrogen bons
DNA polymerase III
The builder enzyme, replicates DNA molecules to build a new strand of DNA
Primase
The initializer, makes the primer so DNA polymerase knows where to start replicating
Ligase
Helps DNA molecules glue together
DNA polymerase I
removes and replaces RNA primers with DNA
replisome
A complex of proteins at each replication fork
Topoisomerase
Relaxes super coiling
How does DNA polymerase III remove errors during replication
has exonucleus “proof readers” , and so if it detects an error, it removes the error sequence and replaces with the correct one.
Hayflick limit
no of times that a normal differentiated cell can go into cell division before cell division stops.
What does PCR require.
DNA Template, a supply of four DNA nucleotides dATP, dGTP,dCPT, dTTP, a heat stable DNA polymerase, two different single stranded DNA primers, A buffer solution.
Limitations Of PCR
Can only work on small fragments, need to know about the sequence you want to amplify in advance.
