T/F The inner mito membrane is extremely permeable.
- False, extremely impermeable
* Outer membrane selectively permeable, big things can get through
What tissues are the richest in mitochondria?
•Brain and Muscles
Over 50% of the mito proteins are made in the?
•Nucleus
How is mitochondria like sitting by a fireplace?
- Has a role in warming the body, especially in new borns
- Brown fat high in mito, keep the baby warm
- Firewood similar to burning fuel in mito, in this case glucose derivatives or fatty acids
Reduce NADH and FADH2 bring…
- e-, Vit B and other energy vitamins
- e- handed from one complex to another, within complex 1 there are 5-10 handoffs going down hill, decreasing ∆G more after each handoff
The pH in the intermembrane space is?
•Low
________ is the ultimate e- acceptor.
•Oxygen
Electrons flow in Complex I from NADH through ______ and a series of iron sulfur clusters to ________.
- FMN (flavin mononucleotide)
- Ubiqinone (Q)
- FAD (flavin adenine dinucleotide)
- Flavin proteins are a component of ETC
Complexes are somewhat fixed, Co Q can go between Complexes __ and __, and Cyto C can go between __ and __.
- I and III
- III and IV
- Cytochrome are a heme containing protein, proforin ring structur
∆Gº’ = -nF∆E’
- n = number of e-
- F is faraday’s constant
- ∆E’ is reduction potential
How does brown fat convert e- flow into warmth?
•Protons just pump e- in and back out, don’t yield ATP
What is the role of ATPsynthase?
- When the proton gradient goes through the system, it turns the wheel to make ATP
- Made up of F1 and F0 subunits
What is the role of the F1 subunit?
- Makes the ATP, ATPase portion
* Subunits alpha, beta, gamma, delta, epsilon
What is the role of F0 subunit?
- Protein translocator
* Made up of the b2, a, and c ring subunits
The rotor spins because of _______ coming through the membrane.
•Protons
T/F the spinning rotor spins in a counterclockwise direction.
•True
What are the subunits of the spinning rotor?
- C ring, and gamma/epislon (everything else stationary component)
- Mechanical spinning transduces energy necessary to make ATP
- Whole complex takes energy from downhill movement of protons and makes ATP
What is the driving force of this process to make ATP?
•ADP*
What are the 3 conformations of the ring?
- T = Tight, synthesis ATP
- L = Loose, binds ATP
- O = Open, releases newly formed ATP
