Sodium Citrate Con. Correction
(1.8 x 10^-3) (100-H)(V) = C
Use only if the hct is >55
Routine Light Microscopy Method
% = No. of retics /1000RBCs x 100
PT Formula (INR)
(patients PT/Mean Normal PT)^ISI
Calibrated Miller Disc Method
% = total retics in square A / (total RBCs in Square B x 9) x100
Absolute Retic count
AR= [no of retics% x RBC count (x10^12/L)] / 100 x 1000
may mal
Corrected Retic count
CRC = retics (%) x [HCT (L/L) / 0.45L/L
Reference range for ARC
20 - 115 x 10^9/L
Retic Production Index
Corrected Retic count / Maturation Time in the Peripheral Blood
Hgb
RBC ct x 3
Hct = Hgb x 3
MCH
Hgb / RBC x10
MCV
Hct / RBC x 10
MCHC
Hgb / Hct x 100
Formula for RBC count rules
Ave cell x DF / ACF x depth facy
Absolute count of each cell for Absolute WBC count
WBC diff. count (in dec) x Total WBC count (x10^9)
Corrected WBC Ct
CR WBC ct = (uncontrolled WBc x100) / (nucleated rbcs + 100)
Formula for platelet count (Rees and Ecker)
(total cells counted x df) / (ACF x depth factor)
ACF = 10(0.04) = .4
Platelet formula (Unopette)
(total cells counted x df) / (ACF x depth factor)
ACF = 10(0.04) = .4
WBC ct estimate if 40x magnification is used
Average WBC x 2000
WBC ct estimate if 50x magnification is used
Average WBC x 3000
Platelet ct. estimate
Average platelet per field x 20,000
Platelet ct. estimate (patient has erythrocytosis or increased Hct value)
Average platelets per field x rb ctc / 200 RBCs
Computation for Cyanmethemoglobin methed (beer’s)
(OD of sample/OD of standard) x ( conc. of standard (mg/dL)/100 mg/g) x 251
5mL of Drabkin’s = 1:251
6mL = 1:301
C test = (C standard x A test)/A standard
Corrected Hgb
Hgb - [plasma Hgb x (1-Hct)]
Retic inx / RPI / CRC
Absolute Retic count / Maturation correction