HEMATOCRIT

Question 1

Volume of packed red blood cells that occupies a given volume of whole blood after centrifugation of a blood sample

Also known as ________ or _________

Reported either as _________,_________,__________or __________

Answer 1

Hematocrit

Packed Cell volume or erythrocyte volume fraction

Percentage
Cell volume percent
Volume percent
Liters per liters

Question 2

Plasma composed of (4)

Answer 2

Water
Proteins
Nutrients
Hormones

Question 3

Buffy coat composed of (2)

Answer 3

White blood cell
Platelets

Question 4

Hematocrit composed of (1)

Answer 4

Red blood cells

Question 5

Adam’s microhematocrit method materials:

Specimen used:

Capillary tubes

Sealing clay

Wax

Microhematocrit centrifuge

Microhematocrit reader

Answer 5

Blood anticoagulated with EDTA or heparin or from capillary puncture

Question 6

Two types of capillary tubes:

Blue ring:

Red ring:

Answer 6

Non heparinized, used for EDTA samples

heparinizef, used for skin puncture sample

Question 7

capillary tube size

Length:

Bore:

Field portion:

Answer 7

7 - 7.5 CM

1.2 MM

5 CM

Question 8

Adam’s microhematocrit method procedure:

Fill two plain capillary tubes approximately__________ full with blood anticoagulated with EDTA or heparin

Sealed end of the tube with the colored ring using_______, seal at a _______angle and plug should be at least_______long

Balance the tubes in a micro hematocrit and refuge with the clay and facing outside away from the center, touching the ________

Tighten the head cover on the head centrifuge and includes the top and centrifuge the tubes at________to______ For_______ Minutes

The values of duplicate hematocrit should agree within______

Answer 8

3/4

Non-absorbent ceiling clay ; 90° and 4MM

Rubber gasket

10,000g to 15,000g for 4 - 5 minutes

1%

Question 9

Reference values of hematocrit in conventional unit

Male:

female:

Answer 9

40 to 54%

35 to 49%

Question 10

Reference values of hematocrit in SI unit:

Male:

Female:

Answer 10

0.4 2 0.54 L/ L

0.35 to 0.49 L/ L

Question 11

Sources of error of microhematocrit:

Improper sealing of the capillary tube causes a_________hematocrit reading as a result of leakage of blood during centrifugation

and increase concentration of anticoagulant _______the hematocrit reading

_____or_____ result may occur if the specimen was not mixed properly

Insufficient centrifugation or delay in reading results after centrifugation causes hematocrit reading to_______

Answer 11

Decreased

Decreases

Decreased or Increased

Increase

Question 12

_______should not be included in the hematocrit reading because this falsely elevate the result

Disorders such as ______,_____,_____,_____,____, may cause plasma to be trapped in the RBC layer even if the procedure is performed properly

__________, amount of plasma that still remains in the RBC portion after the micro hematocrit has been spun

Trapping of the plasma causes the microhematocrit be ____to___% higher than the value obtained using automated instruments

Answer 12

Buffy coat

Sickle cell anemia, macrocytic anemia, hypochromic anemia, spherocytosis, thalassemia

Trapped plasma

1% to 3%

Question 13

Automated methods used:

Answer 13

Coulter counter

Autoanalyzer

Question 14

Formula of hematocrit

Answer 14

Hematocrit= RBC x MCV

Question 15

Wintrobe method materials

Specimen used:

Wintrobe tube

Pasteur pipette

centrifuge

Answer 15

Blood anticoagulated with EDTA

Question 16

Winthrop tube size:

length:

Bore:

Answer 16

11.5 CM

3 MM

Question 17

Wintrobe tube 2 calibrations:

0 to 10 (RED):

10 to 0 (WHITE):

Answer 17

Used for erythrocyte sedimentation rate

Used for hematocrit

Question 18

Anticoagulant of choice in Wintrobe method

Answer 18

Double oxalate

Question 19

Wintrobe method procedure:

fill the wintrobe tube with blood using a_________

Centrifuge the tube at____for______

Wintrobe method hematocrit formula:

Answer 19

Pasteur pipette

3000 RPM for 30 minutes

Question 20

Wintrobe method hematocrit formula:

Answer 20

Hematocrit= (height of packed RBC / height of wholeblood) x 100

Question 21

Rule of three formula:

Answer 21

RBC x 3 = Hgb

Hgb x 3 = Hct +/-3