LE CELL

Question 1

A _________ or _________ that has engulfed the denatured nuclear material of another cell

Answer 1

Neutrophil or Macrophage

Question 2

A neutrophil or macrophage that has engulfed the natural nuclear material

Answer 2

LE cells

Question 3

The denatured material is an absorbed______

Answer 3

Haematoxylin body

Question 4

Basophilic particle is also called an

Answer 4

NE body

Question 5

The autoimmune disease most commonly associated with LE cells

Answer 5

Systemic lupus erythematosus

Question 6

Lupus erythematosus is also found in

Answer 6

Similar connective tissue disorders

Question 7

Patients with systemic lupus erythematosus have an________ that causes____and_______certain blood cell nuclei in vitro

Answer 7

Abnormal plasma protein

Swelling and breakdown

Question 8

Type of leukocyte that attracts phagocytic cells which engulfs the nuclear mass

Answer 8

Segmented neutrophils

Question 9

Phagocytes and inclusion material is termed as

Answer 9

LE cell

Question 10

A Chronic sometimes fatal disease of unknown etiology

Answer 10

Systemic lupus erythematosus

Question 11

Peculiar skin eruption across nose and cheeks

Answer 11

Butterfly rash

Question 12

Diagnosis of SLE depends on the___[

Answer 12

Demonstration of LE cell

Question 13

Hematologic finding of SLE (3)

Answer 13

Leukopenia

Thrombocytopenia

Elevated sedimentation rate

Question 14

Process of Leukocytes are broken down in vitro allowing the abnormal plasma protein to react on altered nuclear material

Answer 14

Rotary bead method

Question 15

Step that enhances nuclear deterioration and Phagocytosis

Answer 15

Incubation

Question 16

The inclusion body with leukocyte is _______ and________ this feature distinguishes the true LE cell from tart cell

It is called_____

Answer 16

Homogenous and has no chromatin pattern

Nucleophagocytosis

Question 17

It contains engulfed damaged nucleus, usually that of_________ which still contains recognizable chromatin pattern and distinct nuclear membrane

Answer 17

Tart cell

Lymphocyte

Question 18

Type of cell that forms a tart cell

Answer 18

Monocyte or histiocyte

Question 19

The type of blood specimen used in the procedure

Answer 19

Heparinized blood

Question 20

The anticoagulant used in the sample preparation

Answer 20

Heparin

Question 21

The tube used for centrifugation of blood

Answer 21

Wintrobe tube

Question 22

Add____ glass beads into the tube

Answer 22

5

Question 23

Rotate blood sample on a__________ at______for_______

Answer 23

Blood mixing rotor

50 rpm for 30 minutes

Question 24

Place the tube at_____for_____

Answer 24

37°C for 10 minutes

Question 25

Centrifuge wintrobe tube at____for_____

Answer 25

3000 rpm for 30 minutes

Question 26

Remove________ and_______ carefully

Answer 26

Hemolyzed serum and buffy coat

Question 27

Make smears of_____

Answer 27

Buffy coat

Question 28

The chemical used to fix the smear

Answer 28

Methanol

Question 29

The stain used for identifyingBa LE cells

Answer 29

Wright’s stain

Question 30

The ideal temperature for performing the LE cell test and the process may be hastend by incubation at________

Answer 30

22°C 37°C

Question 31

Percentage of LE cell test considered positive

Answer 31

2% to 30%

Question 32

Minimum number of LE cell seen in 15 minutes search to consider a smear positive

Answer 32

10 or more

Question 33

Negative findings on LE cell testing exclude

Answer 33

Diagnosis of SLE

Question 34

LE cells are also positive in (7)

Answer 34

Rheumatoid arthritis Chronic hepatitis Scleroderma Dermatomyositis Polyarteritis nodosa Acquired hemolytic anemia Hodgkin disease