lab 7th Flashcards

(30 cards)

1
Q

specificity allows a certain group of organisms to [..] while [..] others

A

grow
inhibiting

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2
Q

sensitivity is regarding the [..], when it changes, and if it turns [..] with it

A

pH
color

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3
Q

does staphylococcus aureus have growth or no growth in the MSA plate?

MSA = mannitol salt agar plate

A

growth

staphylococcus aureus has aurea in the salt environment where it is hard to survive so it does have “aura”

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4
Q

since staphylococcus aureus does have growth in the MSA agar plate what color does it turn?

A

yellow

yellow aura = thing of for gold

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5
Q

MSA

since staphylococcus aureas has growth and is yellow, what does that mean?

grew in the mannitol salt agar plate and is yellow

A
  • salt tolerant
  • ferments mannitol

staphylococcus aureas has aura so it does grow in salt which is hard, and since it is yellow for golden aurea it ferments mannitol

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6
Q

MSA & MC

does staphylococcus epidermidis have growth or no growth in the MSA and MC agar plates?

A
  • MSA = growth
  • MC = no growth

epidermidis = skin = skin is salty, not gram -

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7
Q

MSA

since staphylococcus epidermidis grows in salty environments, MSA, what color does it become ?

A

pink

staphylococcus epidermidis = skin = skin is salty

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8
Q

since staphylococcus epidermidis does not grow in MacConkey agar plates, what color is it?

A

N/A

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9
Q

since staphylococcus epidermidis grows in MSA plates and is pink, what is the interpretation ?

A
  • salt tolerant
  • no mannitol fermentation (since it is not yellow)

think of pink skin for the color pink of the organism

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10
Q

Since staphylococcus epidermidis does not grow on Mac Conkey plate and has no color change what does this interpret?

A
  • gram positive
  • crystal violet & bile salts inhibit growth
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11
Q

does streptococcus pyogenes have growth or no growth in the MSA plate?

A

no growth

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12
Q

since streptococcus pyogenes has no growth in the MSA plate, what is the color

A

N/A

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13
Q

since streptococcus pyogenes have no growth in the MSA plate and no color indication, what is the interpretation

A

not salt tolerant

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14
Q

does escherichia coli have growth or no growth in the MC agar plate?

A

growth

mac CONKEY = mac DONKEY = donkeys poop = escherichia coli

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15
Q

since escherichia coli does grow on MC plates, what color does it become

A

hot pink

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16
Q

since escherichia coli grows in the Mac Conkey plate and is hot pink, what is the interpretation

A
  • gram negative
  • can ferment lactose (colliform)
17
Q

does pseudomonas aeruginosa have growth or no growth in the MC plate?

18
Q

since pseudomonas aeruginosa has growth in the MC agar plate, what color does it become?

A

greenish yellow

19
Q

since the pseudomonas aeruginosa does have growth in the MC plate and is greenish yellow, what does this interpret?

A
  • gram negative
  • does not ferment lactose
20
Q

color changes based on [..] indicator

21
Q

aerotolerance is the organisms ability to tolerate [..]

22
Q

aerotolerance

what does obligate aerobe mean

A

requires oxygen to survive
growth on top of flask since its where most oxygen is at

23
Q

what does obligate anaerobe mean

A

requires anoxygenic environemnts to survive
growth at botto of flask since its where least oxygen is located

24
Q

what does facilitative anaerobes mean

A

prefer oxygen, but can survive without it
most growth on top but some scatted throughout rest of liquid

25
what does perotolerant anaerobes mean
no preference growth all scattered | p for perotolerant for no preference
26
what do microaerophiles mean
requires less than atmospheric levels of oxygen growth in middle of liquid
27
what is the purpose of a nitrate reduction test
to identify if an organism can reduce nitrate and how
28
what is the purpose of the catalase test
to identify if an organism has the ezyme catalase and can breakdown hydrogen peroxide (H2O2) into water (H2O) and oxygen (O2)
29
overall steps to the catalase test
- add bacteria directly to the slide and then add a few drops of catalase reactant (H2O2) - positive reaction; bubbles means the organism has the catalase enzyme and can breakdown H2O2 into H2O and O2 - negative reaction; no bubbling/reaction
30
why do we see bubbles in the catalase test
because of the release of gas (oxygen) & water