in the aerotolerance test what did escherichia coli, show as?
facultative
growth primarily on top but also spread out throughout since it is facil
what is an organism that showed facultative growth in the aerotolerance test ?
escherichia coli & staphylococcus aureus
what did staphylococcus aureus show up as in the aerotolerance test?
facultative
growth on top and some throughout
what did the streptococcus pyogene show up as in the aerotolerance test?
aerotolerant
tolerable to all oxygen levels, growth throughout all, genes
what is the organism that showed up as aerotolerant in the aerotolerance test?
streptococcus pyogene
what did clostridium sporogenes show up as in the aerotolerance test?
obligate anaerobe
clostridium sporogenes = gene spores = die = anaerobe
what organism shows up as obligate anaerobe in the aerotolerance test
clostridium sporogenes
what is the first step in the nitrate reduction test
check durham for a LARGE bubble, if present, (+) reaction for nitrate reduction and nitrogen gas production
what should we look for when stopping at step 1 for
a large bubble, if there is none, move to step 2
what is step 2 in the nitrate reduction test
add 8 drops nitrate reagent A, then add 8 drops of nitrate reagent B, mix. a red color change is (+) nitrate reduction, reducing nitrate -> nitrite via nitrate reductase
what signs should we look for to stop at step 2, if not to move to the next step
if changes color red after 8 drops of nitrate reagent A and nitrate reagent B, a red signals nitrate reduction, so reducing nitrate to nitrite via nitrate reductase
if no red color change, move to step 3
what is step 3 in the nitrate reduction test
add a scoup of zinc powder, zinc reacts with nitrate to produce a red color; (-) for nitrate reduction
no red, (+) nitrate reduction, by some other means than whata has been previously described
what step did escherichia coli turn red after
step 2
what did it mean when escherichia coli turned red after step 2
positive for nitrate -> nitrite
after what step did streptococcus pyogenes turn red
red after step 3
what does it mean when streptococcus pyogenes turned red after step 3
negative nitrate reduction
what is the purpose of the durham tube?
to capture gas if it is produced
is the phenol red (PR) broth selective or differential ?
differential media
(phenol red pH indicator for fermentatino of many things)
what is the purpose of the phenol red (PR) broth?
to identify if an organism cna ferment a particular carbohydrate with or without gas production
what does the durham tube do?
capture gas, if it is produced from fermentation
what are the two organisms used for the phenol red (PR) broth , and coagulase test
- staphylococcus aureus
- staphylococcus epidermidis
what is the purpose of the coagulase test?
used to identify staphylococcus aureus
what is coagulase
an enzyme, and virulence factor
what is the purpose of the starch hydrolysis test
to identify if an organism has amylase exoenzymes and can breakdown starch outside of the cell & bring smaller parts in to utilize as nutrients
