in the coagulase test, the positive organism is solid for the organism
in coagulase there is a s and an l for solid and liquid
staphylococcus aureus
s equals positive
aureus is positive
in the coagulase test the organism that is negative is liquid and is called
coagulase has an l and an s for liquid and solid
staphylococcus epidermidis
l for liquid who negative
aurea is for positives epidermidis, liquid is
in the phenol red, staphylococcus epidermidis (a negative liquid) is the color [..], the color is negative
red
think of the epidermidis as the blood by being red and a liquid red=wron
in phenol red, a positive is yellow in fermentation for the organism
staphylococcus aureus
if its yellow check for gas production : no bubble N/A gas
when iodine was used in the starch hydrolysis there is a halo for the organism
bacillus subtilis
halo as in a a bat flying down with moon shinning
batillus subtilis
the organism that was negative in the starch hydrolysis test was [..] [..] because it did not have a halo
staphylococcus epidermidis
in the antibiotic screening both plates included [..] [..]
streptococcus pneumonae
in the antibiotic screening test, the [..] was resistant
bacitracin
bat + tranny = bacitracin
in the antibiotic screening quiz, the organism that was susceptible was the [..]
optochin
opp for optochin. op is positive for susceptibility
the zone of inhibition should be greater than or equal to [..] [..] to be susceptible
14 mm
IMViC
stands for
Indole
methyl red
i stands for nothing
Voges and citrate
the indole which is the I for IMViC uses a [..] agar
SIM
S- H2S production
I - indole production from tryptophan
M - motile (growth outside of stab line )
for the indole, the I in IMViC, what do you do to the agar
you deep stab using a needle
MV which is methyl red purposes are
methyl red = identifying mixed acid fermentation (MR-VP broth)
MV in IMViC the v that stands for voges ‘s purpose is
proskaver identify glucose fermentation, specifically with 2,3 - butanediol production
methyl red and voges in IMViC are incoluated in our test or two different agars?
one
citrate in IMViC the c that stands for citrate ‘s purpose is
to identify if an organism can utilize citrate as a carbon source (only source in the media)
citrate has c and so does carbon
in the methyl red and voges test the bacteria is added into flask with a [..]
loop
in the citrate test in the IMViC test the bacteria is added [..] and then when pulling out [..]
slanted
zig-zag
decarboxylase (ornithine) takes about [..] hours
96
the decarboxylase (ornithine) test identifies if an organism can [..] a specific amino acid (ornithine)
decarboxylate
in the decarboxylase (ornithine) test the agar requires addition of [..]” mineral oil after you add bacteria. the minderal is to promote an [..] environment
1/4”
anaerobic
why does the decarboxylase (ornithine) require so much time inoculated
because of the anaerobic environment
what are the species used for
escherichia coli
salmonella typamirium
