Lab Lectures

Question 1

Symptoms of cystic fibrosis

Answer 1

Presistent cough with mucus
Repeated lung infection
Wheezing
Poor growth
Blocked airways

Question 2

Molecular clonning steps

Answer 2

1.isolating DNA from species
2.inserting DNA into cloning vector (recombination)
3. Inserting recombinant vector into host cell (transformation)
4. Using host celll to make copies of DNA (replication)

Question 3

Restriction enzyme

Answer 3

Naturally in bacterias
Defence mechanism
Endonuclease proteins- break phosphordiaster bonds

Question 4

Multiple types of CFTR gene mutations

Answer 4

1. No protein formation (cl-ion channel)
2. No traffic
3. No function
4. Less function
   5.less protein
5. Less stable

Question 5

Phases of bacterial growth

Answer 5

Lag , exponential, stationary and death

Question 6

Lag phase

Answer 6

Bacterias grow — adjust to environemtn

Question 7

Exponential

Answer 7

Pop doubles every 20 mins — taking up nuteirnts

Question 8

Stationary phase

Answer 8

Rate of cell division= death of cells

Question 9

Death phase

Answer 9

Death > celll divison
Nutrients exhausted

Question 10

Enzyme unit

Answer 10

Ammount of enzyme that catlyses the conversion of one micromole of substrate per min under specified conditions of assay method