Substrate requirement for polymerase
Template
Primer— dna pol cant initiate dna synthesis bt itself
Free 3’ OH -
How does the catalytic site in dna pol help catalyse reax
Addition of dNPTS
Specific aa seq side chains in pol
†wo mg +2
Can rNPTs be incorparated during dna replication
No, 2’ OH grp causes steric hinderance
Palm like active site twists dna template so only 2 nt is in the active site
T/F
False— only one nt
How is error corrected during DNA replication
Exonuclease
All dna have associated exonuclease activity
T/F
FALSE
Not all dna pol have associated exonuclease activity
Replication occurs in bidirectional fork
T/F
TRUE
5’——>3’
3’<——5’
rna pol can synthesis rna without a primer but dna pol cant synthesis dna w/o primer
True
Leading strand
Continous strand 5’——>3’
OKAZAKI fragments
Lagging strand
What synthesis rna primers
Primase 11+- 1
At the end of replication how are rna primers removed
Exonuclease
Dna ligase
Seals nicks
Dna helicase
Unwinds dna
Toposiomerase
Prevents supercoiling
Positive super coil is good
T/F
False
Positive is bad
Negative is good
Ssbp
Single strand binding proteins
Prevents strand reannealing
Replisome
Polymerase + accessory enzymes
Sliding clamp and clamp loader -keeps pol on dna
What ensures that both strands are being processed in sync
Lagging strand has to be in loop
