1
Q
RNA- seq
A
Isloate RNA—-RNA to cDNA—-fragment cDNA —sequence— map sequence reads
2
Q
What primers can we use when sequencing RNA library prep
A
RNA- cDNA
Primers—-1. Polt T tail 2. Random hexamers
3
Q
Pros of using poly T primers
A
Select mature mRNA only
4
Q
Cons of using polly T primers
A
Not all genes have poly A in mRNA
May not get info on splicing
5
Q
Types of RNA-seq library kits
A
.mRNA— based on poly T
Ribo depletion — no rRNA
MicroRNAs
6
Q
Exone exone junctions
A
Shows what exons areincluded or excluded from transcripts
Help infer is splicing has occured
